Browsing by Subject "DNA recognition"
Now showing items 1-10 of 10
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Directed self-assembly of trimeric DNA-bindingchiral miniprotein helicates
(Frontiers Media, 2018)We propose that peptides are highly versatile platforms for the precise design of supramolecular metal architectures, and particularly, for the controlled assembly of helicates. In this context, we show that the bacteriophage ... -
Fluorescence-Labeled Bis-benzamidines as Fluorogenic DNA Minor-Groove Binders: Photophysics and Binding Dynamics
(WILEY-VCH Verlag GmbH, 2014-11-21)In recent decades there has been great interest in the design of highly sensitive sequence-specific DNA binders. The eligibility of the binder depends on the magnitude of the fluorescence increase upon binding, related to ... -
Light-Controlled Cellular Internalization and Cytotoxicity of Nucleic Acid-Binding Agents: Studies in Vitro and in Zebrafish Embryos
(Wiley, 2016)We synthesized octa‐arginine conjugates of DNA‐binding agents (bisbenzamidine, acridine and Thiazole Orange) and demonstrated that their DNA binding and cell internalization can be inhibited by appending a (negatively ... -
Nickel-promoted recognition of long DNA sites by designed peptide derivatives
(WILEY-VCH Verlag GmbH, 2016-08-03)We describe the synthesis of designed peptidic modules that self-assemble in specific DNA sequences of 12 base pairs in the presence of NiII salts. The modules consist of modified fragments of transcription factors that ... -
Reversible supramolecular assembly at specific DNA sites: Ni-promoted, bivalent DNA binding with designed peptide and bipyridylbisbenzamidine components
(WILEY-VCH Verlag GmbH, 2014-07-15)At specific DNA sites, nickel(II) salts promote the assembly of designed components, namely a bis(histidine)-modified peptide that is derived from a bZIP transcription factor and a bis(benzamidine) unit that is equipped ... -
Selective DNA-Binding by Designed Bisbenzamidine-Homeodomain Chimeras
(WILEY-VCH Verlag GmbH, 2014-04-24)We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor-groove DNA binders. The hybrid featuring the sequence of the native protein ... -
Sequence‐Selective DNA Recognition with Peptide–Bisbenzamidine Conjugates
(Wiley, 2013)Transcription factors (TFs) are specialized proteins that play a key role in the regulation of genetic expression. Their mechanism of action involves the interaction with specific DNA sequences, which usually takes place ... -
Sequence‐Specific DNA Recognition with Designed Peptides
(Wiley, 2018)Inspired by natural transcription factors (TFs), researchers have explored the potential of artificial peptides for the recognition of specific DNA sequences, developing increasingly sophisticated systems that not only ... -
Temporary Electrostatic Impairment of DNA Recognition: Light‐Driven DNA Binding of Peptide Dimers
(Wiley, 2012)Appending negatively charged Glu8 tails to a peptide dimer derived from the GCN4 transcription factor leads to an effective suppression of its DNA binding. The specific DNA recognition can be restored by irradiation with ...