Instituto de Acuiculturahttp://hdl.handle.net/10347/29722024-03-28T16:51:50Z2024-03-28T16:51:50ZDetection of different Betanodavirus genotypes in wild fish from Spanish Atlantic coastal waters (Galicia, northwestern Spain)Vázquez Salgado, LucíaOlveira Hermida, José GabrielPereira Dopazo, CarlosBandín Matos, María Isabelhttp://hdl.handle.net/10347/332552024-03-21T09:21:45Z2023-01-01T00:00:00ZDetection of different Betanodavirus genotypes in wild fish from Spanish Atlantic coastal waters (Galicia, northwestern Spain)
Vázquez Salgado, Lucía; Olveira Hermida, José Gabriel; Pereira Dopazo, Carlos; Bandín Matos, María Isabel
Objective
The nervous necrosis virus (NNV; genus Betanodavirus) is an aquatic pathogen that is responsible for a neurological disease affecting marine fish. Despite its almost worldwide distribution, global warming could favor the spread of NNV to new areas, highlighting the importance of conducting epidemiological surveys on both wild and farmed marine fish species. In this study, we assessed NNV prevalence in wild fish caught along the Galician Atlantic coast.
Methods
In total, 1277 fish were analyzed by reverse transcription real-time polymerase chain reaction.
Result
Twenty two (1.72%) of those fish tested positive for NNV, including two species in which the pathogen had not yet been reported.
Conclusion
The reassortant RGNNV/SJNNV (red-spotted grouper NNV/striped jack NNV) was detected in 55% of NNV-positive individuals, while the remaining 45% harbored the SJNNV-type genome. Moreover, from European Pilchard Sardina pilchardus and Atlantic Mackerel Scomber scombrus, we isolated four reassortant strains that carried amino acid mutations at key sites related to NNV–host interaction.
2023-01-01T00:00:00ZGenetic diferentiation of a critically endangered population of the limpet Patella candei candei d’Orbigny, 1840, in the Canary IslandsQuinteiro Vázquez, JavierGonzález-Lorenzo, GustavoHernández Reyes, DailosQuinteiro, LaraHerrera Pérez, RogelioMartínez Barrio, JuanGonzález Ramos, Antonio JuanRey Méndez, ManuelGonzález Henríquez, Nieveshttp://hdl.handle.net/10347/292002022-09-03T02:02:36Z2022-01-01T00:00:00ZGenetic diferentiation of a critically endangered population of the limpet Patella candei candei d’Orbigny, 1840, in the Canary Islands
Quinteiro Vázquez, Javier; González-Lorenzo, Gustavo; Hernández Reyes, Dailos; Quinteiro, Lara; Herrera Pérez, Rogelio; Martínez Barrio, Juan; González Ramos, Antonio Juan; Rey Méndez, Manuel; González Henríquez, Nieves
The adoption of measures to protect the viability of threatened populations should be supported by empirical data identifying appropriate conservation units and management strategies. The global population of the majorera limpet, P. candei candei d’Orbigny, 1840, is restricted to the Macaronesian islands in the NE Atlantic, including near-to-extinct and healthy populations in Fuerteventura and Selvagens, respectively. The taxonomic position, genetic diversity and intra- and interspecific relationships of these populations are unclear, which is hindering the implementation of a recovery plan for the overexploited majorera limpet on Fuerteventura. In this study, ddRAD-based genome scanning was used to overcome the limitations of mitochondrial DNA-based analysis. As a result, P. candei candei was genetically differentiated from the closely related P. candei crenata for the first time. Moreover, genetic differentiation was detected between P. candei candei samples from Selvagens and Fuerteventura, indicating that translocations from the healthy Selvagens source population are inadvisable. In conclusion, the majorera limpet requires population-specific management focused on the preservation of exceptional genetic diversity with which to face future environmental challenges
2022-01-01T00:00:00ZVibrio neptunius produces piscibactin and amphibactin and both siderophores contribute significantly to virulence for clamsGalvis Serrano, Néstor FabiánAgeitos, LucíaRodríguez González, JaimeJiménez González, CarlosBarja Pérez, Juan LuisBalado Dacosta, MiguelLemos Ramos, Manuel Luishttp://hdl.handle.net/10347/272282023-07-10T06:10:55Z2021-01-01T00:00:00ZVibrio neptunius produces piscibactin and amphibactin and both siderophores contribute significantly to virulence for clams
Galvis Serrano, Néstor Fabián; Ageitos, Lucía; Rodríguez González, Jaime; Jiménez González, Carlos; Barja Pérez, Juan Luis; Balado Dacosta, Miguel; Lemos Ramos, Manuel Luis
Vibrio neptunius is an inhabitant of mollusc microbiota and an opportunistic pathogen causing disease outbreaks in marine bivalve mollusc species including oysters and clams. Virulence of mollusc pathogenic vibrios is mainly associated with the production of extracellular products. However, siderophore production is a common feature in pathogenic marine bacteria but its role in fitness and virulence of mollusc pathogens remains unknown. We previously found that V. neptunius produces amphibactin, one of the most abundant siderophores in marine microbes. In this work, synthesis of the siderophore piscibactin was identified as the second siderophore produced by V. neptunius. Single and double mutants in biosynthetic genes of each siderophore system, piscibactin and amphibactin, were constructed in V. neptunius and their role in growth ability and virulence was characterized. Although the High Pathogenicity Island encoding piscibactin is a major virulence factor in vibrios pathogenic for fish, the V. neptunius wild type did not cause mortality in turbot. The results showed that amphibactin contributes more than piscibactin to bacterial fitness in vitro. However, infection challenges showed that each siderophore system contributes equally to virulence for molluscs. The V. neptunius strain unable to produce any siderophore was severely impaired to cause vibriosis in clams. Although the inactivation of one of the two siderophore systems (either amphibactin or piscibactin) significantly reduced virulence compared to the wild type strain, the ability to produce both siderophores simultaneously maximised the degree of virulence. Evaluation of the gene expression pattern of each siderophore system showed that they are simultaneously expressed when V. neptunius is cultivated under low iron availability in vitro and ex vivo. Finally, the analysis of the distribution of siderophore systems in genomes of Vibrio spp. pathogenic for molluscs showed that the gene clusters encoding amphibactin and piscibactin are widespread in the Coralliilyticus clade. Thus, siderophore production would constitute a key virulence factor for bivalve molluscs pathogenic vibrios
2021-01-01T00:00:00ZThe Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen Vibrio neptuniusGalvis Serrano, Nestor FabiánBarja Pérez, Juan LuisBalado Dacosta, MiguelLemos Ramos, Manuel Luishttp://hdl.handle.net/10347/268102023-07-10T06:10:57Z2021-01-01T00:00:00ZThe Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen Vibrio neptunius
Galvis Serrano, Nestor Fabián; Barja Pérez, Juan Luis; Balado Dacosta, Miguel; Lemos Ramos, Manuel Luis
Vibrio neptunius is an important pathogen of bivalve mollusks worldwide. Several metalloproteases have been described as virulence factors in species of Vibrio that are pathogenic to bivalves, but little is known about the contribution of these potential virulence factors to Vibrio neptunius pathogenesis. In silico analysis of the genome of V. neptunius strain PP-145.98 led to the identification of two hitherto uncharacterized chromosomal loci encoding a probable vibriolysin-like metalloprotease and a putative collagenase, which were designated VnpA and ColA, respectively. Single defective mutants of each gene were obtained in V. neptunius PP-145.98, and the phospholipase, esterase and collagenase activities were studied and compared with those of the wild-type strain. The results showed that the single inactivation of vnpA resulted in a 3-fold reduction in phospholipase/esterase activity. Inactivation of colA reduced the collagenase activity by 50%. Finally, infection challenges performed in oyster larvae showed that ΔvnpA and ΔcolA—single mutant strains of V. neptunius—are between 2–3-fold less virulent than the wild-type strain. Thus, the present work demonstrates that the production of both VnpA and ColA is required for the full virulence of the bivalve pathogen V. neptunius
2021-01-01T00:00:00Z