Now showing items 1-18 of 18

    • Anion Recognition as a Supramolecular Switch of Cell Internalization 

      Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; Rodríguez Couceiro, José; Nitschke, Jonathan R.; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (American Chemical Society, 2016)
      The cell internalization of designed oligoarginine peptides equipped with six glutamic acid residues and an anionic pyranine at the N-terminus is triggered upon addition of a supramolecular host. This host binds specifically ...
    • A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves 

      Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; García Fandiño, Rebeca; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Royal Society of Chemistry, 2016-02-05)
      We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which ...
    • A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves 

      Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; García Fandiño, Rebeca; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Royal Society of Chemistry, 2016)
      We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which ...
    • DNA-binding miniproteins based on zinc fingers. Assessment of the interaction using nanopores 

      Rodríguez Villar, Jéssica; Learte Aymamí, Soraya; Mosquera Mosquera, Jesús; Celaya, Garbiñe; Rodríguez Larrea, David; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Royal Society of Chemistry, 2018)
      Obtaining artificial proteins that mimic the DNA binding properties of natural transcription factors could open new ways of manipulating gene expression at will. In this context it is particularly interesting to develop ...
    • Intracellular deprotection reactions mediated by palladium complexes equipped with designed phosphine ligands 

      Martínez Calvo, Miguel; Rodríguez Couceiro, José; Destito, Paolo; Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; Mascareñas Cid, José Luis (American Chemical Society, 2018-06-01)
      Discrete palladium(II) complexes featuring purposely designed phosphine ligands can promote depropargylation and deallylation reactions in cell lysates. These complexes perform better than other palladium sources, which ...
    • Nickel-promoted recognition of long DNA sites by designed peptide derivatives 

      Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (WILEY-VCH Verlag GmbH, 2016-08-03)
      We describe the synthesis of designed peptidic modules that self-assemble in specific DNA sequences of 12 base pairs in the presence of NiII salts. The modules consist of modified fragments of transcription factors that ...
    • Reversible Control of Protein Corona Formation on Gold Nanoparticles Using Host–Guest Interactions 

      Mosquera Mosquera, Jesús; García, Isabel; Henriksen-Lacey, Malou; Martínez Calvo, Miguel; Dhanjani, Mónica; Mascareñas Cid, José Luis; Liz-Marzán, Luis M. (ACS Publications, 2020)
      When nanoparticles (NPs) are exposed to biological media, proteins are adsorbed, forming a so-called protein corona (PC). This cloud of protein aggregates hampers the targeting and transport capabilities of the NPs, thereby ...
    • Reversible supramolecular assembly at specific DNA sites: Ni-promoted, bivalent DNA binding with designed peptide and bipyridylbisbenzamidine components 

      Sánchez López, Mateo Isidro; Mosquera Mosquera, Jesús; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (WILEY-VCH Verlag GmbH, 2014-07-15)
      At specific DNA sites, nickel(II) salts promote the assembly of designed components, namely a bis(histidine)-modified peptide that is derived from a bZIP transcription factor and a bis(benzamidine) unit that is equipped ...
    • Ruthenation of Non‐stacked Guanines in DNA G‐Quadruplex Structures: Enhancement of c‐MYC Expression 

      Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; Rodríguez Couceiro, José; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Wiley, 2016)
      Guanine quadruplexes (GQs) are compact four‐stranded DNA structures that play a key role in the control of a variety of biological processes, including gene transcription. Bulky ruthenium complexes featuring a bipyridine, ...
    • Ruthenium bipyridyl complexes as photocleavable dimerizers: deactivation of DNA-binding peptides using visible light 

      Mosquera Mosquera, Jesús; Sánchez López, Mateo Isidro; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Royal Society of Chemistry, 2014-07-28)
      We report a photolabile biselectrophilic Ru(II) complex that can be used for homo- or heterodimerization of cysteine-containing peptides. The resulting dimers can be efficiently disassembled by long-wavelength light. As ...
    • Selective DNA-Binding by Designed Bisbenzamidine-Homeodomain Chimeras 

      Mosquera Mosquera, Jesús; Rodríguez Villar, Jéssica; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (WILEY-VCH Verlag GmbH, 2014-04-24)
      We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor-groove DNA binders. The hybrid featuring the sequence of the native protein ...
    • Sequence-selective DNA binding with cell-permeable oligoguanidinium-peptide conjugates 

      Mosquera Mosquera, Jesús; Sánchez López, Mateo Isidro; Valero, Julián; Mendoza, Javier de; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Royal Society of Chemistry, 2015-02-18)
      Conjugation of a short peptide fragment from a bZIP protein to an oligoguanidinium tail results in a DNA-binding miniprotein that selectively interacts with composite sequences containing the peptide-binding site next to ...
    • Stimuli-Responsive DNA Binding by Synthetic Systems 

      Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; Learte Aymamí, Soraya; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (American Chemical Society, 2020)
      Conspectus: DNA is the molecule responsible for the storage and transmission of the genetic information in living organisms. The expression of this information is highly regulated. In eukaryotes, it is achieved mainly at ...
    • Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides 

      Mosquera Mosquera, Jesús; Jiménez Balsa, Adrián; Dodero, Verónica Isabel; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Nature Publishing Group, 2013)
      One of the strategies used by nature to regulate gene expression relies on the stimulicontrolled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether ...
    • Supramolecular caging for cytosolic delivery of anionic probes 

      Fernández Caro, Héctor; Lostalé Seijo, Irene; Martínez Calvo, Miguel; Mosquera Mosquera, Jesús; Mascareñas Cid, José Luis; Montenegro García, Javier (Royal Society of Chemistry, 2019)
      The cytosolic delivery of hydrophilic, anionic molecular probes and therapeutics is a major challenge in chemical biology and medicine. Herein, we describe the design and synthesis of peptide–cage hybrids that allow an ...
    • Synthetic peptides caged on histidine residues with a bisbipyridyl ruthenium(II) complex that can be photolyzed by visible light 

      Mosquera Mosquera, Jesús; Sánchez López, Mateo Isidro; Mascareñas Cid, José Luis; Vázquez Sentís, Marco Eugenio (Royal Society of Chemistry, 2015-01-08)
      We report a light-sensitive histidine building block for Fmoc/tBu solid-phase peptide synthesis in which the imidazole side chain is coordinated to a ruthenium complex. We have applied this building block for the synthesis ...
    • The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments 

      Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; Rodríguez Couceiro, José; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Royal Society of Chemistry, 2015)
      We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide ...
    • The ββα fold of zinc finger proteins as a “natural” protecting group. Chemoselective synthesis of a DNA-binding zinc finger derivative 

      Rodríguez Villar, Jéssica; Mosquera Mosquera, Jesús; Vázquez Vázquez, Olalla; Vázquez Sentís, Marco Eugenio; Mascareñas Cid, José Luis (Royal Society of Chemistry, 2014)
      We report the selective modification of cysteine residues engineered in peptides that have two additional cysteine residues as part of a Cys2His2 zinc finger motif. The chemoselective modification is achieved, thanks to ...





      Harvesters:Useful links:
      Universidade de Santiago de Compostela | Teléfonos: +34 881 811 000 e +34 982 820 000 | Contact Us | Send Feedback