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dc.contributor.authorMartínez Costas, José Manuel
dc.contributor.authorFernández Trillo, Francisco
dc.contributor.authorMontenegro García, Javier
dc.contributor.authorGranja Guillán, Juan Ramón
dc.contributor.authorPriegue Caamaño, Juan Manuel
dc.contributor.authorCrisan, Daniel
dc.identifier.citationJ. M. Priegue, D. N. Crisan, J. Martínez-Costas, J. R. Granja, F. Fernandez-Trillo, J. Montenegro, Angew. Chem. Int. Ed. 2016, 55, 7492
dc.descriptionNOTICE:This is the peer-reviewed version of the following article:Juan M. Priegue, Daniel N. Crisan, José Martínez-Costas, Juan R. Granja, Francisco Fernandez-Trillo, and Javier Montenegro (2016),“In situ Functionalized Polymers for siRNA Delivery ; Angew. Chem. Int. Ed., 55, 7492–7495 [doi: 10.1002/anie.201601441]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving
dc.description.abstractA new method is reported herein for screening the biological activity of functional polymers across a consistent degree of polymerization and in situ, that is, under aqueous conditions and without purification/isolation of candidate polymers. In brief, the chemical functionality of a poly(acryloyl hydrazide) scaffold was activated under aqueous conditions using readily available aldehydes to obtain amphiphilic polymers. The transport activity of the resulting polymers can be evaluated in situ using model membranes and living cells without the need for tedious isolation and purification steps. This technology allowed the rapid identification of a supramolecular polymeric vector with excellent efficiency and reproducibility for the delivery of siRNA into human cells (HeLa-EGFP). The reported method constitutes a blueprint for the high-throughput screening and future discovery of new polymeric functional materials with important biological applications
dc.description.sponsorshipRoyal Society. Grant Number: IE130688 Spanish Ministry of Economy and Competitiveness. Grant Numbers: CTQ2014-59646-R, CTQ2013-43264-R, BFU2013-43513-R Birmingham Science City European Regional Development Fund Royal Society. Grant Number: RG140273 University of Birmingham MEC MINECO ERC. Grant Number: DYNAP-677786
dc.publisherWILEY-VCH Verlag GmbH
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-43513-R/ES/REOVIRUS AVIAR: FACTORES DE VIRULENCIA Y NUEVAS DIANAS TERAPEUTICAS
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-59646-R/ES/NUEVOS DISEÑOS CON CONTROL TOPOLOGICO DE PEPTIDOS PENETRANTES EN CELULAS
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2013-43264-R/ES/NUEVAS TERAPIAS SUPRAMOLECULARES DIRIGIDAS A LAS MEMBRANAS LIPIDICAS Y BASADAS EN NANOESTRUCTURAS AUTOENSAMBLADAS
dc.rights© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.subjectLipid bilayer membranes
dc.subjectsiRNA delivery
dc.subjectSupramolecular chemistry
dc.titleIn situ functionalized polymers for siRNA delivery
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica

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