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dc.contributor.author | Silva, Marisa |
dc.contributor.author | Rey López, Verónica |
dc.contributor.author | Botana López, Ana María |
dc.contributor.author | Vasconcelos, Vitor |
dc.contributor.author | Botana López, Luis Miguel |
dc.date.accessioned | 2017-10-19T11:19:21Z |
dc.date.available | 2017-10-19T11:19:21Z |
dc.date.issued | 2015-12-30 |
dc.identifier.citation | Silva, M.; Rey, V.; Botana, A.; Vasconcelos, V.; Botana, L. Determination of Gonyautoxin-4 in Echinoderms and Gastropod Matrices by Conversion to Neosaxitoxin Using 2-Mercaptoethanol and Post-Column Oxidation Liquid Chromatography with Fluorescence Detection. Toxins 2016, 8, 11. |
dc.identifier.issn | 2072-6651 |
dc.identifier.uri | http://hdl.handle.net/10347/15860 |
dc.description.abstract | Paralytic Shellfish Toxin blooms are common worldwide, which makes their monitoring crucial in the prevention of poisoning incidents. These toxins can be monitored by a variety of techniques, including mouse bioassay, receptor binding assay, and liquid chromatography with either mass spectrometric or pre- or post-column fluorescence detection. The post-column oxidation liquid chromatography with fluorescence detection method, used routinely in our laboratory, has been shown to be a reliable method for monitoring paralytic shellfish toxins in mussel, scallop, oyster and clam species. However, due to its high sensitivity to naturally fluorescent matrix interferences, when working with unconventional matrices, there may be problems in identifying toxins because of naturally fluorescent interferences that co-elute with the toxin peaks. This can lead to erroneous identification. In this study, in order to overcome this challenge in echinoderm and gastropod matrices, we optimized the conversion of Gonyautoxins 1 and 4 to Neosaxitoxin with 2-mercaptoethanol. We present a new and less time-consuming method with a good recovery (82.2%, RSD 1.1%, n = 3), requiring only a single reaction step |
dc.description.sponsorship | This research was partially funded by the Portuguese Fundation of Science and Technology (FCT) project UID/Multi/04423/2013 and by the projects MARBIOTECH (reference NORTE-07-0124-FEDER-000047) within the Scientific Resaerch and Technological Development (SR&TD) Integrated Program. MARVALOR—Building research and innovation capacity for improved management and valorizationof marine resources, supported by the Programa Operacional Regional do Norte (ON.2-O Novo Norte) and NOVOMAR (reference 0687-NOVOMAR-1-P), supported by the European Regional Development Fund. Marisa Silva also acknowledges FCT for the grant SFRH/BD/73269/2010. The spanish research leading to these results has received funding from the following European Fund for Economic and Regional Development (FEDER) cofunded-grants. From Centro para el Desarrollo Tecnológico Industrial (CDTI) and Technological Funds, supported by Ministerio de Economía y Competitividad, AGL2012-40185-CO2-01, AGL2014-58210-R, and Consellería de Cultura, Educación e Ordenación Universitaria, GRC2013-016. From CDTI under India&Spain Innovating Program (ISIP) Programme, Spain, IDI-20130304 APTAFOOD. From the European Union’s Seventh Framework Programme managed by REA—Research Executive Agency (FP7/2007-2013) under grant agreement 312184 PHARMASEA |
dc.language.iso | eng |
dc.publisher | MDPI |
dc.rights | © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/) |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.subject | Paralytic shellfish poisoning |
dc.subject | Toxins |
dc.subject | Post-column oxidation fluorescence |
dc.subject | Interfering matrix peaks |
dc.subject | Thiol compounds |
dc.subject.classification | Materias::Investigación::32 Ciencias médicas::3214 Toxicología |
dc.title | Determination of Gonyautoxin-4 in Echinoderms and Gastropod Matrices by Conversion to Neosaxitoxin Using 2-Mercaptoethanol and Post-Column Oxidation Liquid Chromatography with Fluorescence Detection |
dc.type | journal article |
dc.identifier.doi | 10.3390/toxins8010011 |
dc.relation.publisherversion | http://doi.org.10.3390/toxins8010011 |
dc.type.hasVersion | VoR |
dc.rights.accessRights | open access |
dc.contributor.affiliation | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
dc.contributor.affiliation | Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía |
dc.description.peerreviewed | SI |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/312184 |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-58210-R/ES/EVALUACION DE LA SEGURIDAD ALIMENTARIA DE PRODUCTOS PESQUEROS ASOCIADA A LA PRESENCIA DE TOXINAS MARINAS DE NUEVA APARICION EN AGUAS EUROPEAS |
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