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dc.contributor.authorRodríguez Filgueiras, Inés
dc.contributor.authorAlfonso Rancaño, María Amparo
dc.contributor.authorAlonso López, Eva
dc.contributor.authorRubiolo Gaytán, Juan Andrés
dc.contributor.authorRoel Sánchez, María
dc.contributor.authorVlamis, Aristidis
dc.contributor.authorKatikou, Panagiota
dc.contributor.authorJackson, Stephen A
dc.contributor.authorMargassery, Lekha Menon
dc.contributor.authorDobson, Alan
dc.contributor.authorBotana López, Luis Miguel
dc.identifier.citationRodríguez, I., Alfonso, A., Alonso, E., Rubiolo, J. A., Roel, M., Vlamis, A., ... & Botana, L. M. (2017). The association of bacterial C9-based TTX-like compounds with Prorocentrum minimum opens new uncertainties about shellfish seafood safety. Scientific reports, 7, 40880
dc.description.abstractIn 2012, Tetrodotoxin (TTX) was identified in mussels and linked to the presence of Prorocentrum minimum (P. minimum) in Greece. The connexion between TTX and P. minimum was further studied in this paper. First, the presence of TTX-producer bacteria, Vibrio and Pseudomonas spp, was confirmed in Greek mussels. In addition these samples showed high activity as inhibitors of sodium currents (INa). P. minimum was before associated with neurotoxic symptoms, however, the nature and structure of toxins produced by this dinoflagellate remains unknown. Three P. minimum strains, ccmp1529, ccmp2811 and ccmp2956, growing in different conditions of temperature, salinity and light were used to study the production of toxic compounds. Electrophysiological assays showed no effect of ccmp2811 strain on INa, while ccmp1529 and ccmp2956 strains were able to significantly reduce INa in the same way as TTX. In these samples two new compounds, m/z 265 and m/z 308, were identified and characterized by liquid chromatography tandem high-resolution mass spectrometry. Besides, two TTX-related bacteria, Roseobacter and Vibrio sp, were observed. These results show for the first time that P. minimum produce TTX-like compounds with a similar ion pattern and C9-base to TTX analogues and with the same effect on INa
dc.description.sponsorshipInés Rodríguez is supported by a fellowship from Subprograma de Formación de Personal Investigador MINECO (AGL2012-40185-CO2-01), Spain. The research leading to these results has received funding from the following FEDER cofunded-grants. From CDTI and Technological Funds, supported by Ministerio de Economía y Competitividad, AGL2012-40185-CO2-01, AGL2014-58210-R, and Consellería de Cultura, Educación e Ordenación Universitaria, GRC2013-016. From CDTI under ISIP Programme, Spain, IDI-20130304 APTAFOOD. From the European Union’s Seventh Framework Programme managed by REA – Research Executive Agency (FP7/2007-2013) under grant agreement 312184 PHARMASEA.
dc.publisherNature Publishing Group
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-58210-R/ES/EVALUACION DE LA SEGURIDAD ALIMENTARIA DE PRODUCTOS PESQUEROS ASOCIADA A LA PRESENCIA DE TOXINAS MARINAS DE NUEVA APARICION EN AGUAS EUROPEAS
dc.rights© 2017, the authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
dc.subjectMass spectrometry
dc.subjectPatch clamp
dc.subjectPCR-based techniques
dc.titleThe association of bacterial C9-based TTX-like compounds with Prorocentrum minimum opens new uncertainties about shellfish seafood safety
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica

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