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dc.contributor.authorTomás Gamasa, María
dc.contributor.authorMartínez Calvo, Miguel
dc.contributor.authorRodríguez Couceiro, José
dc.contributor.authorMascareñas Cid, José Luis
dc.date.accessioned2017-10-21T13:12:01Z
dc.date.available2017-10-21T13:12:01Z
dc.date.issued2016-09-07
dc.identifier.citationToma´s-Gamasa, M. et al. Transition metal catalysis in the mitochondria of living cells. Nat. Commun. 7:12538 doi: 10.1038/ncomms12538 (2016)
dc.identifier.urihttp://hdl.handle.net/10347/16015
dc.description.abstractThe development of transition metal catalysts capable of promoting non-natural transformations within living cells can open significant new avenues in chemical and cell biology. Unfortunately, the complexity of the cell makes it extremely difficult to translate standard organometallic chemistry to living environments. Therefore, progress in this field has been very slow, and many challenges, including the possibility of localizing active metal catalysts into specific subcellular sites or organelles, remain to be addressed. Herein, we report a designed ruthenium complex that accumulates preferentially inside the mitochondria of mammalian cells, while keeping its ability to react with exogenous substrates in a bioorthogonal way. Importantly, we show that the subcellular catalytic activity can be used for the confined release of fluorophores, and even allows selective functional alterations in the mitochondria by the localized transformation of inert precursors into uncouplers of the membrane potential
dc.description.sponsorshipWe are thankful for the support given by the Spanish grant SAF2013-41943-R, the Xunta de Galicia (GRC2013-041 and 2015-CP082), the ERDF and the European Research Council (Advanced Grant No. 340055). M.T.G. thanks the Ministerio de Economía y Competitividad for the Postdoctoral fellowship. M.M.C. thanks Ministerio de Economía y Competitividad for the Juan de la Cierva-Incorporacio´n fellowship (IJCI-2014-19326).
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/340055
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/IJCI-2014-19326/ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2013-41943-R/ES/HERRAMIENTAS SINTETICAS EN QUIMICA BIOLOGICA. NUEVAS ESTRATEGIAS MOLECULARES PARA EL TRATAMIENTO Y DIAGNOSTICO DE CANCERES
dc.rights© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.subjectCatalysis
dc.subjectChemical biology
dc.subjectOrganic chemistry
dc.titleTransition metal catalysis in the mitochondria of living cells
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1038/ncomms12538
dc.relation.publisherversionhttp://dx.doi.org/10.1038/ncomms12538
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn2041-1723
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.description.peerreviewedSI


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