A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves
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| Title: | A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves |
| Author: | Rodríguez Villar, Jéssica Mosquera Mosquera, Jesús García Fandiño, Rebeca Vázquez Sentís, Marco Eugenio Mascareñas Cid, José Luis |
| Affiliation: | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares Universidade de Santiago de Compostela. Departamento de Química Orgánica |
| Date of Issue: | 2016-02-05 |
| Publisher: | Royal Society of Chemistry |
| Citation: | J. Rodríguez, J. Mosquera, R. García-Fandiño, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2016, 7, 3298–3303 |
| Abstract: | We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG) |
| Publisher version: | http://dx.doi.org/10.1039/C6SC00045B |
| URI: | http://hdl.handle.net/10347/16016 |
| DOI: | 10.1039/c6sc00045b |
| ISSN: | 2041-6539 |
| Rights: | © The author(s) 2016. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence |
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