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dc.contributor.authorJuanes Carrasco, Marisa
dc.contributor.authorLostalé Seijo, Irene
dc.contributor.authorGranja Guillán, Juan Ramón
dc.contributor.authorMontenegro García, Javier
dc.date.accessioned2018-05-02T17:07:12Z
dc.date.available2018-10-27T01:00:09Z
dc.date.issued2018-04-27
dc.identifier.citationJuanes, M., Lostalé-Seijo, I., Granja, J., & Montenegro, J. (2018). Supramolecular Recognition and Selective Protein Uptake by Peptide Hybrids. Chemistry - A European Journal. http://dx.doi.org/10.1002/chem.201800706
dc.identifier.urihttp://hdl.handle.net/10347/16690
dc.descriptionThis manuscript has been accepted after peer review and appears as anAccepted Article online prior to editing, proofing, and formal publicationof the final Version of Record (VoR). This work is currently citable byusing the Digital Object Identifier (DOI) given below. The VoR will bepublished online in Early View as soon as possible and may be differentto this Accepted Article as a result of editing. Readers should obtainthe VoR from the journal website shown below when it is publishedto ensure accuracy of information. The authors are responsible for thecontent of this Accepted Article
dc.description.abstractThe intracellular transport of exogenous proteins has emerged as one of the most promising methodologies for biotechnology and chemical biology. Current protein delivery is mainly approached by liposome encapsulation, translational fusion and ionic/hydrophobic non‐covalent aggregation with transporting molecular vehicles. We here introduce the concept of supramolecular recognition and selective transport of proteins by peptide hybrid materials. We have designed a helical amphiphilic cationic peptide that bears two orthogonal alkoxyamines for the precise anchoring of protein ligands. After the attachment of these protein ligands, the peptide showed a high binding affinity for its protein target (i.e. mannose/Concanavalin A, Biotin/Streptavidin). The resulting peptide/protein hybrids were taken up by human cells such as HeLa and HepG2. The concept described in this manuscript could potentially be adapted, through the appropriate choice of ligands, to the transport of other proteins with suitable supramolecular binding motifs
dc.description.sponsorshipThis work was partially supported by the Spanish Agencia Estatal de Investigación (AEI) [CTQ2014-59646-R, CTQ2013-43264-R, SAF2017-89890-R], the Xunta de Galicia (ED431G/09, ED431C 2017/25 and 2016-AD031) and the ERDF. We thank Pili Canoa (CACTI, UVIGO) for assistance with the SPR measurements. M.J. received a F.P.I. fellowship from MINECO (CTQ2014-59646-R). J.M. received a Ramón y Cajal (RYC-2013-13784), an ERC Starting Investigator Grant (DYNAP- 677786) and a Young Investigator Grant from the Human Frontier Science Research Program (RGY0066/2017)
dc.language.isoeng
dc.publisherWiley
dc.rights© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
dc.subjectSupramolecular systems
dc.subjectMembranes
dc.subjectPeptides
dc.subjectPenetrating peptides
dc.subjectAmphiphiles
dc.subjectProtein
dc.subjectTransport
dc.titleSupramolecular Recognition and Selective Protein Uptake by Peptide Hybrids
dc.typejournal article
dc.identifier.doi10.1002/chem.201800706
dc.relation.publisherversionhttps://doi.org/10.1002/chem.201800706
dc.type.hasVersionAM
dc.identifier.essn1521-3765
dc.rights.accessRightsopen access
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.description.peerreviewedSI
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/677786
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-59646-R/ES/NUEVOS DISEÑOS CON CONTROL TOPOLOGICO DE PEPTIDOS PENETRANTES EN CELULAS
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2013-43264-R/ES/NUEVAS TERAPIAS SUPRAMOLECULARES DIRIGIDAS A LAS MEMBRANAS LIPIDICAS Y BASADAS EN NANOESTRUCTURAS AUTOENSAMBLADAS
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-89890-R/ES/PEPTIDOS HIBRIDOS PARA EL TRANSPORTE SELECTIVO Y ENTREGA DE PROTEINAS TERAPEUTICAS
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2013-13784/ES


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