Nanomedicines for cell reprogramming in tissue engineering and immunomodulation
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Title: | Nanomedicines for cell reprogramming in tissue engineering and immunomodulation |
Author: | Martínez Ledo, Adriana |
Advisor: | Alonso Fernández, María José García Fuentes, Marcos |
Affiliation: | Universidade de Santiago de Compostela. Facultade de Farmacia Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
Subject: | Nanomedicine | Biomaterials | Tissue engineering | Cancer immunotherapy | |
Date of Issue: | 2018 |
Abstract: | The main goal of this thesis has been the development of various RNA-based therapies to modulate cell phenotypes in two different disease contexts: cancer and tissue engineering. Given the poor cell-permeability of naked RNA molecules, as well as their short half-life in biorelevant media, a critical aspect of the work has been the design and development of biomaterial-based systems intended to overcome these limitations. On the one hand, we developed a nanocarrier capable of co-allocating RNAi and chemokines to revert the tumor immunosuppression mediated by myeloid derived suppressor cells. On the other hand, we investigated a new concept of gene-activated matrices (GAMs) activated with mRNA-encoded transcription factors (TFs) to direct stem cell chondrogenic and myogenic specification. We finally optimized GAM mechanics in order to enhance TF transfection and cell differentiation driven by TF overexpression. |
URI: | http://hdl.handle.net/10347/16770 |
Rights: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
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- Área de Ciencias da Saúde [1016]
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