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dc.contributor.authorPinto, Luiz F.
dc.contributor.authorCorrea, Juan
dc.contributor.authorZhao, Libo
dc.contributor.authorRiguera Vega, Ricardo
dc.contributor.authorFernández Megía, Eduardo
dc.date.accessioned2018-07-04T10:57:58Z
dc.date.available2018-07-04T10:57:58Z
dc.date.issued2018-03-12
dc.identifier.citationPinto, L., Correa, J., Zhao, L., Riguera, R., & Fernandez-Megia, E. (2018). Fast NMR Screening of Macromolecular Complexes by a Paramagnetic Spin Relaxation Filter. ACS Omega, 3, 3, 2974-2983. doi: 10.1021/acsomega.7b02074
dc.identifier.urihttp://hdl.handle.net/10347/16947
dc.description.abstractThe paramagnetic spin relaxation filter is described for the rapid NMR screening of intermolecular interactions between ligands and macromolecular anionic receptors with large transverse relaxation enhancements (R2p). The addition of micromolar concentrations of Gd3+ to the mixture produces the immediate broadening/suppression of the NMR signals of interacting species while leaving unaffected those of noncompetitive binders (one-dimensional and two-dimensional experiments). The method is highly sensitive, unveiling interactions that are too weak to generate changes in chemical shifts or relaxation times. It is operationally very simple and hence, it is amenable to ready implementation by nonspecialists. Examples of application such as detecting the formation of interpolymer complexes, cyclodextrin host–guest interactions, and the screening of DNA ligands are included that demonstrate the reliability and broad applicability of the method
dc.description.sponsorshipThis work was financially supported by the Spanish Ministry of Economy, Industry, and Competitiveness (MINECO)(CTQ2015-69021-R), the Xunta de Galicia (GRC2014/040 and Centro Singular de Investigacion de Galicia Accreditation 2016-2019, ED431G/09), and the European Union (European Regional Development Fund-ERDF)
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-69021-R/ES/MICELAS DENDRITICAS EN TERAPIA Y DIAGNOSTICO: CONTROL DE LA ARQUITECTURA Y ACTIVIDAD A PARTIR DE LA ESTRUCTURA MOLECULAR
dc.rightsThis is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes (https://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
dc.titleFast NMR Screening of Macromolecular Complexes by a Paramagnetic Spin Relaxation Filter
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1021/acsomega.7b02074
dc.relation.publisherversionhttps://doi.org/10.1021/acsomega.7b02074
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn2470-1343
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.description.peerreviewedSI


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