Exploring the efficiency of gallic acid-based dendrimers and their block copolymers with PEG as gene carriers
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10347/16974
Files in this item
Metadata
Title: | Exploring the efficiency of gallic acid-based dendrimers and their block copolymers with PEG as gene carriers |
Author: | Fuente Freire, María de la Raviña Fernández, Manuela Sousa Hervés, Ana Correa, Juan Riguera Vega, Ricardo Fernández Megía, Eduardo Sánchez Barreiro, Alejandro Alonso Fernández, María José |
Affiliation: | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas Universidade de Santiago de Compostela. Departamento de Farmacia e Tecnoloxía Farmacéutica Universidade de Santiago de Compostela. Departamento de Química Orgánica |
Subject: | Dendrimers | Gene delivery | HEK-293T | In vitro | Polyethylene glycol | |
Date of Issue: | 2012-07-20 |
Publisher: | Future Science |
Citation: | de la Fuente, M., Raviña, M., Sousa-Herves, A., Correa, J., Riguera, R., & Fernandez-Megia, E. et al. (2012). Exploring the efficiency of gallic acid-based dendrimers and their block copolymers with PEG as gene carriers. Nanomedicine, 7(11), 1667-1681. doi: 10.2217/nnm.12.51 |
Abstract: | The synthesis of a new family of amino-functionalized gallic acid-triethylene glycol (GATG) dendrimers and their block copolymers with polyethylene glycol (PEG) has recently being disclosed. In addition, these dendrimers have shown potential for gene delivery applications, as they efficiently complex nucleic acids and form small and homogeneous dendriplexes. On this basis, the present study aimed to explore the interaction of the engineered dendriplexes with blood components, as well as their stability, cytotoxicity and ability to enter and transfect mammalian cells. Results show that GATG dendrimers can form stable dendriplexes, protect the associated pDNA from degradation, and are biocompatible with HEK-293T cells and erythrocytes. More importantly, dendriplexes are effectively internalized by HEK-293T cells, which are successfully transfected. Besides, PEGylation has a marked influence on the properties of the resulting dendriplexes. While PEGylated GATG dendrimers have improved biocompatibility, the long PEG chains limit their uptake by HEK-293T cells, and thus, their ability to transfect them. As a consequence, the degree of PEGylation in dendriplexes containing dendrimer/block copolymer mixtures emerges as an important parameter to be modulated in order to obtain an optimized stealth formulation able to effectively induce the expression of the encoded protein |
Publisher version: | https://doi.org/10.2217/nnm.12.51 |
URI: | http://hdl.handle.net/10347/16974 |
DOI: | 10.2217/nnm.12.51 |
Rights: | © 2012 Future Science |
Collections
-
- CIMUS-Artigos [188]
- CIQUS-Artigos [394]
- FTF-Artigos [201]
- QO-Artigos [356]