| dc.contributor.author | González-Vera, Juan A. |
| dc.contributor.author | Bouzada, David |
| dc.contributor.author | Bouclier, Céline |
| dc.contributor.author | Vázquez Sentís, Marco Eugenio |
| dc.contributor.author | Morris, May C. |
| dc.date.accessioned | 2018-07-13T12:39:22Z |
| dc.date.available | 2018-07-13T12:39:22Z |
| dc.date.issued | 2017 |
| dc.identifier.citation | González-Vera, J., Bouzada, D., Bouclier, C., Eugenio Vázquez, M., & Morris, M. (2017). Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity. Chemical Communications, 53(45), 6109-6112. doi: 10.1039/c6cc09948c |
| dc.identifier.issn | 1359-7345 |
| dc.identifier.uri | http://hdl.handle.net/10347/17023 |
| dc.description.abstract | We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety |
| dc.description.sponsorship | This work was funded by the CNRS (Centre National de la Recherche Scientifique) and a Marie-Curie fellowship EC-FP7 Framework (PIEF-GA-2013-623151) supporting JAGV. CB was funded by the INCA (PRTK-2014). Financial support from the Spanish MINECO (CTQ2015-70698-R), the Xunta de Galicia (Centro singular de investigacio´n de Galicia accreditation 2016–2019), and the European Union (European Regional Development Fund – ERDF), are gratefully acknowledged |
| dc.language.iso | eng |
| dc.publisher | Royal Society of Chemistry |
| dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-70698-R/ES/SISTEMAS SUPRAMOLECULARES PARA RECONOCIMIENTO BIOMOLECULAR: HELICATOS PEPTIDICOS Y RECEPTORES OLIGOMERICOS AUTOENSAMBLADOS |
| dc.rights | © The Royal Society of Chemistry 2017 |
| dc.title | Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity |
| dc.type | info:eu-repo/semantics/article |
| dc.identifier.DOI | 10.1039/C6CC09948C |
| dc.relation.publisherversion | https://doi.org/10.1039/C6CC09948C |
| dc.type.version | info:eu-repo/semantics/acceptedVersion |
| dc.identifier.e-issn | 1364-548X |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess |
| dc.contributor.affiliation | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares |
| dc.contributor.affiliation | Universidade de Santiago de Compostela. Departamento de Química Orgánica |
| dc.description.peerreviewed | SI |
