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dc.contributor.authorGonzález-Vera, Juan A.
dc.contributor.authorBouzada, David
dc.contributor.authorBouclier, Céline
dc.contributor.authorVázquez Sentís, Marco Eugenio
dc.contributor.authorMorris, May C.
dc.date.accessioned2018-07-13T12:39:22Z
dc.date.available2018-07-13T12:39:22Z
dc.date.issued2017
dc.identifier.citationGonzález-Vera, J., Bouzada, D., Bouclier, C., Eugenio Vázquez, M., & Morris, M. (2017). Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity. Chemical Communications, 53(45), 6109-6112. doi: 10.1039/c6cc09948c
dc.identifier.issn1359-7345
dc.identifier.urihttp://hdl.handle.net/10347/17023
dc.description.abstractWe describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety
dc.description.sponsorshipThis work was funded by the CNRS (Centre National de la Recherche Scientifique) and a Marie-Curie fellowship EC-FP7 Framework (PIEF-GA-2013-623151) supporting JAGV. CB was funded by the INCA (PRTK-2014). Financial support from the Spanish MINECO (CTQ2015-70698-R), the Xunta de Galicia (Centro singular de investigacio´n de Galicia accreditation 2016–2019), and the European Union (European Regional Development Fund – ERDF), are gratefully acknowledged
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-70698-R/ES/SISTEMAS SUPRAMOLECULARES PARA RECONOCIMIENTO BIOMOLECULAR: HELICATOS PEPTIDICOS Y RECEPTORES OLIGOMERICOS AUTOENSAMBLADOS
dc.rights© The Royal Society of Chemistry 2017
dc.titleLanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1039/C6CC09948C
dc.relation.publisherversionhttps://doi.org/10.1039/C6CC09948C
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.e-issn1364-548X
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.description.peerreviewedSI


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