Critical aggregation concentration for the formation of early Amyloid-β (1–42) oligomers
Title: | Critical aggregation concentration for the formation of early Amyloid-β (1–42) oligomers
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Author: | Novo Rodríguez, María de la Merced
Freire Rodríguez, Sonia
Al-Soufi, Wajih
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Affiliation: | Universidade de Santiago de Compostela. Departamento de Química Física
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Subject: | Biophysical chemistry | Peptides | Protein aggregation | |
Date of Issue: | 2018-01-29
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Publisher: | Nature Publishing Group
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Citation: | Novo, M., Freire, S., & Al-Soufi, W. (2018). Critical aggregation concentration for the formation of early Amyloid-β (1–42) oligomers. Scientific Reports, 8(1). doi: 10.1038/s41598-018-19961-3
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Abstract: | The oligomers formed during the early steps of amyloid aggregation are thought to be responsible for the neurotoxic damage associated with Alzheimer’s disease. It is therefore of great interest to characterize this early aggregation process and the aggregates formed, especially for the most significant peptide in amyloid fibrils, Amyloid-β(1–42) (Aβ42). For this purpose, we directly monitored the changes in size and concentration of initially monomeric Aβ42 samples, using Fluorescence Correlation Spectroscopy. We found that Aβ42 undergoes aggregation only when the amount of amyloid monomers exceeds the critical aggregation concentration (cac) of about 90 nM. This spontaneous, cooperative process resembles surfactants self-assembly and yields stable micelle-like oligomers whose size (≈50 monomers, R h ≈ 7–11 nm) and elongated shape are independent of incubation time and peptide concentration. These findings reveal essential features of in vitro amyloid aggregation, which may illuminate the complex in vivo process |
Publisher version: | https://doi.org/10.1038/s41598-018-19961-3 |
URI: | http://hdl.handle.net/10347/17067
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DOI: | 10.1038/s41598-018-19961-3 |
E-ISSN: | 2045-2322
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Rights: | © 2018, the authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional
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