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dc.contributor.authorMéndez Ardoy, Alejandro
dc.contributor.authorLostalé Seijo, Irene
dc.contributor.authorMontenegro García, Javier
dc.date.accessioned2018-09-13T06:51:04Z
dc.date.available2019-03-03T02:00:08Z
dc.date.issued2018-09-03
dc.identifier.citationMéndez Ardoy, A., Lostalé-Seijo, I., & Montenegro, J. (2018). Where in the cell is our cargo? Current methods to study intracellular cytosolic localization. Chembiochem. doi: 10.1002/cbic.201800390
dc.identifier.urihttp://hdl.handle.net/10347/17307
dc.descriptionNOTICE: This is the Accepted Version of the following article: Méndez Ardoy, A., Lostalé-Seijo, I., & Montenegro, J. (2018). Where in the cell is our cargo? Current methods to study intracellular cytosolic localization. Chembiochem. doi: 10.1002/cbic.201800390 © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
dc.description.abstractThe internalization and delivery of active substances into cells is a field of growing interest for chemical biology and therapeutics. As we move from small‐molecule based drugs towards bigger cargos, such as antibodies, enzymes, nucleases or nucleic acids, the development of efficient delivery systems becomes critical for their practical application. Different strategies and synthetic carriers have been developed including cationic lipids, gold nanoparticles, polymers, cell‐penetrating peptides, protein surface modification, etc. However, all these methodologies still present limitations related to the precise targeting of the different intracellular compartments and, in particular, the difficult access to the cellular cytosol. Additionally, the precise quantification of the cellular uptake of a molecule is not enough to demonstrate delivery and/or functional activity. Therefore, methods to determine the cellular distribution of cargos and carriers are of critical importance to identify the barriers that are blocking the activity. In this mini‐review, we survey the different techniques that can be currently used to track and monitor the subcellular localization of the synthetic molecules that we deliver inside cells
dc.description.sponsorshipWe acknowledge funding from the Spanish Agencia Estatal de Investigación (AEI) [CTQ2014-59646-R, SAF2017-89890-R], the Xunta de Galicia (ED431G/09, ED431C 2017/25 and 2016-AD031) and the ERDF. A. M.-A. received a MCIF from the EC (GLYCONANOPEP-750248). J. M. received a Ramón y Cajal (RYC-2013-13784), an ERC Starting Investigator Grant (DYNAP-677786) and a Young Investigator Grant from the Human Frontier Science Research Program (RGY0066/2017)
dc.language.isoeng
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/677786
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-89890-R/ES/PEPTIDOS HIBRIDOS PARA EL TRANSPORTE SELECTIVO Y ENTREGA DE PROTEINAS TERAPEUTICAS
dc.rights© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
dc.subjectProteins
dc.subjectDrug delivery
dc.subjectFluorophores
dc.subjectMicroscopy
dc.subjectCells
dc.titleWhere in the cell is our cargo? Current methods to study intracellular cytosolic localization
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1002/cbic.201800390
dc.relation.publisherversionhttps://doi.org/10.1002/cbic.201800390
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.e-issn1439-7633
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.description.peerreviewedSI


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