The coagulation system helps control infection caused by the ciliate parasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.)
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Título: | The coagulation system helps control infection caused by the ciliate parasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.) |
Autor/a: | Blanco Abad, Verónica Noia Guldrís, Manuel Valle Cao, Alejandra Fontenla Iglesias, Francisco Folgueira López, Iria Felipe González, Ana Paula de Pereiro González, Patricia Leiro Vidal, José Manuel Lamas Fernández, Jesús |
Centro/Departamento: | Universidade de Santiago de Compostela. Departamento de Bioloxía Funcional Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía Universidade de Santiago de Compostela. Instituto de Acuicultura |
Palabras chave: | Fish | Philasterides dicentrarchi | Coagulation system | Neutrophils | Plasma | Complement | |
Data: | 2018-06-20 |
Editor: | Elsevier |
Cita bibliográfica: | Blanco-Abad V, Noia M, Valle A, Fontenla F, Folgueira I, De Felipe AP, Pereiro P, Leiro J, Lamas J. (2018). The coagulation system helps control infection caused by the ciliate parasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.). Dev Comp Immunol. 87:147-156. doi: 10.1016/j.dci.2018.06.001 |
Resumo: | Many studies have shown that coagulation systems play an important role in the defence against pathogens in invertebrates and vertebrates. In vertebrates, particularly in mammals, it has been established that the coagulation system participates in the entrapment of pathogens and activation of the early immune response. However, functional studies investigating the importance of the fish coagulation system in host defence against pathogens are scarce. In the present study, injection of turbot (Scopthalamus maximus) with the pathogenic ciliate Philasterides dicentrarchi led to the formation of macroscopic intraperitoneal clots in the fish. The clots contained abundant, immobilized ciliates, many of which were lysed. We demonstrated that the plasma clots immobilize and kill the ciliates in vitro. To test the importance of plasma clotting in ciliate killing, we inhibited the process by adding a tetrapeptide known to inhibit fibrinogen/thrombin clotting in mammals. Plasma tended to kill P. dicentrarchi slightly faster when clotting was inhibited by the tetrapeptide, although the total mortality of ciliates was similar. We also found that kaolin, a particulate activator of the intrinsic pathway in mammals, accelerates plasma clotting in turbot. In addition, PMA-stimulated neutrophils, living ciliates and several ciliate components such as cilia, proteases and DNA also displayed procoagulant activity in vitro. Injection of fish with the ciliates generated the massive release of neutrophils to the peritoneal cavity, with formation of large aggregates in those fish with live ciliates in the peritoneum. We observed, by SEM, numerous fibrin-like fibres in the peritoneal exudate, many of which were associated with peritoneal leukocytes and ciliates. Expression of the CD18/CD11b gene, an integrin associated with cell adhesion and the induction of fibrin formation, was upregulated in the peritoneal leukocytes. In conclusion, the findings of the present study show that P. dicentrarchi induces the formation of plasma clots and that the fish coagulation system may play an important role in immobilizing and killing this parasite |
Descrición: | This is the accepted manuscript of the following article: Blanco-Abad, V., Noia, M., Valle, A., Fontenla, F., Folgueira, I., & De Felipe, A. et al. (2018). The coagulation system helps control infection caused by the ciliate
parasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.). Developmental & Comparative Immunology, 87, 147-156. doi: 10.1016/j.dci.2018.06.001 |
Versión do editor: | https://doi.org/10.1016/j.dci.2018.06.001 |
URI: | http://hdl.handle.net/10347/18167 |
DOI: | 10.1016/j.dci.2018.06.001 |
ISSN: | 0145-305X |
Dereitos: | © 2018 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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