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dc.contributor.authorMallo Seijas, Natalia
dc.contributor.authorFelipe González, Ana Paula de
dc.contributor.authorFolgueira López, Iria
dc.contributor.authorSueiro Benavides, Rosa Ana
dc.contributor.authorLamas Fernández, Jesús
dc.contributor.authorLeiro Vidal, José Manuel
dc.date.accessioned2019-01-30T08:29:02Z
dc.date.available2019-01-30T08:29:02Z
dc.date.issued2017-01-04
dc.identifier.citationMallo, N. , DeFelipe, A. P., Folgueira, I. , Sueiro, R. A., Lamas, J. and Leiro, J. M. (2017), Combined antiparasitic and anti‐inflammatory effects of the natural polyphenol curcumin on turbot scuticociliatosis. J Fish Dis, 40: 205- 217. doi:10.1111/jfd.12503
dc.identifier.issn1365-2761
dc.identifier.urihttp://hdl.handle.net/10347/18168
dc.descriptionThis is the peer reviewed version of the following article: Mallo, N. , DeFelipe, A. P., Folgueira, I. , Sueiro, R. A., Lamas, J. and Leiro, J. M. (2017), Combined antiparasitic and anti‐inflammatory effects of the natural polyphenol curcumin on turbot scuticociliatosis. J Fish Dis, 40: 205217. doi:10.1111/jfd.12503, which has been published in final form at https://doi.org/10.1111/jfd.12503. This article may be used for noncommercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
dc.description.abstractThe histiophagous scuticociliate Philasterides dicentrarchi is the aetiological agent of scuticociliatosis, a parasitic disease of farmed turbot. Curcumin, a polyphenol from Curcuma longa (turmeric), is known to have antioxidant and anti-inflammatory properties. We investigated the in vitro effects of curcumin on the growth of P. dicentrarchi and on the production of pro-inflammatory cytokines in turbot leucocytes activated by parasite cysteine proteases. At 100 μm, curcumin had a cytotoxic effect and completely inhibited the growth of the parasite. At 50 μm, curcumin inhibited the protease activity of the parasite and expression of genes encoding two virulence-associated proteases: leishmanolysin-like peptidase and cathepsin L-like. At concentrations between 25 and 50 μm, curcumin inhibited the expression of S-adenosyl-L-homocysteine hydrolase, an enzyme involved in the biosynthesis of the amino acids methionine and cysteine. At 100 μm, curcumin inhibited the expression of the cytokines tumour necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) produced in turbot leucocytes activated by parasite proteases. Results show that curcumin has a dual effect on scuticociliatosis: an antiparasitic effect on the catabolism and anabolism of ciliate proteins, and an anti-inflammatory effect that inhibits the production of proinflammatory cytokines in the host. The present findings suggest the potential usefulness of this polyphenol in treating scuticociliatosis
dc.description.sponsorshipThis work was financially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 (PARAFISHCONTROL) and by the Ministerio de Economía y Competitividad (Spain) under grant agreement AGL2014-57125-R
dc.language.isoeng
dc.publisherWiley
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/634429
dc.rights© 2016 John Wiley & Sons Ltd. This article may be used for noncommercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
dc.subjectPhilasterides dicentrarchi
dc.subjectCurcumin
dc.subjectProteases
dc.subjectS-adenosyl-L-homocysteine hydrolase
dc.subjectCathepsin L-like
dc.subjectLeishmanolysin
dc.titleCombined antiparasitic and anti‐inflammatory effects of the natural polyphenol curcumin on turbot scuticociliatosis
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1111/jfd.12503
dc.relation.publisherversionhttps://doi.org/10.1111/jfd.12503
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioloxía Funcional
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Acuicultura
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Investigación e Análises Alimentarias
dc.description.peerreviewedSI


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