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dc.contributor.authorMallo Seijas, Natalia
dc.contributor.authorLamas Fernández, Jesús
dc.contributor.authorFelipe González, Ana Paula de
dc.contributor.authorSueiro Benavides, Rosa Ana
dc.contributor.authorFontenla Iglesias, Francisco
dc.contributor.authorLeiro Vidal, José Manuel
dc.date.accessioned2019-01-30T09:29:06Z
dc.date.available2019-01-30T09:29:06Z
dc.date.issued2016-10
dc.identifier.citationMallo N, Lamas J, de Felipe AP, Sueiro RA, Fontenla F, Leiro JM. (2016). Role of H(+)-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects. Exp Parasitol. 169:59-68. doi: 10.1016/j.exppara.2016.07.012
dc.identifier.issn0014-4894
dc.identifier.urihttp://hdl.handle.net/10347/18174
dc.descriptionThis is the accepted manuscript of the following article: Mallo, N., Lamas, J., de Felipe, A.P., Sueiro, R.A., Fontenla, F. & Leiro, J.M. (2016). Role of H(+)-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects. Experimental Parasitolology, 169, 59-68. doi: 10.1016/j.exppara.2016.07.012
dc.description.abstractThe scuticociliatosis is a very serious disease that affects the cultured turbot, and whose causal agent is the anphizoic and marine euryhaline ciliate Philasterides dicentrarchi. Several protozoans possess acidic organelles that contain high concentrations of pyrophosphate (PPi), Ca(2+) and other elements with essential roles in vesicular trafficking, pH homeostasis and osmoregulation. P. dicentrarchi possesses a pyrophosphatase (H(+)-PPase) that pumps H(+) through the membranes of vacuolar and alveolar sacs. These compartments share common features with the acidocalcisomes described in other parasitic protozoa (e.g. acid content and Ca(2+) storage). We evaluated the effects of Ca(2+) and ATP on H (+)-PPase activity in this ciliate and analyzed their role in maintaining intracellular pH homeostasis and osmoregulation, by the addition of PPi and inorganic molecules that affect osmolarity. Addition of PPi led to acidification of the intracellular compartments, while the addition of ATP, CaCl2 and bisphosphonates analogous of PPi and Ca(2+) metabolism regulators led to alkalinization and a decrease in H(+)-PPase expression in trophozoites. Addition of NaCl led to proton release, intracellular Ca(2+) accumulation and downregulation of H(+)-PPase expression. We conclude that the regulation of the acidification of intracellular compartments may be essential for maintaining the intracellular pH homeostasis necessary for survival of ciliates and their adaptation to salt stress, which they will presumably face during the endoparasitic phase, in which the salinity levels are lower than in their natural environment
dc.description.sponsorshipThis work was financially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 (PARAFISHCONTROL), by the Ministerio de Economía y Competitividad (Spain) under grant agreement AGL2014-57125-R and by grant GPC2014/069 from the Xunta de Galicia (Spain)
dc.language.isoeng
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/634429
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-57125-R/ES/DISEÑO Y ELABORACION DE NUEVAS VACUNAS QUE PERMITAN UNA MAYOR PROTECCION E INOCUIDAD FRENTE A ESCUTICOCILIADOS PARASITOS DEL RODABALLO
dc.rights© 2016 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPhilasterides dicentrarchi
dc.subjectIntracellular pH
dc.subjectCalcium
dc.subjectATP
dc.subjectH+-PPase
dc.subjectOsmoregulation
dc.titleRole of H(+)-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1016/j.exppara.2016.07.012
dc.relation.publisherversionhttps://doi.org/10.1016/j.exppara.2016.07.012
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Acuicultura
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Investigación e Análises Alimentarias
dc.description.peerreviewedSI


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© 2016 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Except where otherwise noted, this item's license is described as  © 2016 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)





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