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dc.contributor.advisorRivas Vázquez, Mª Carmen
dc.contributor.advisorCollado Rodríguez, Manuel
dc.contributor.authorEl Motiam, Ahmed Hassan
dc.date.accessioned2019-02-11T09:32:55Z
dc.date.available2019-02-11T09:32:55Z
dc.date.issued2019
dc.identifier.urihttp://hdl.handle.net/10347/18256
dc.description.abstractThe ribosomal protein L11 integrate different types of stress into a p53-mediated response. Here we analyzed the impact of the ubiquitin-like protein SUMO on the RPL11-MDM2-p53 signaling. We show that SUMO modify RPL11, mutation of all lysine residues in RPL11 did not abolish SUMOylation, suggesting that this conjugation occurs through an alternative non-canonical SUMOylation pathway. We find that SUMO downregulates the conjugation of the ubiquitin-like protein NEDD8 to RPL11 and promotes the translocation of RPL11 outside of the nucleoli. Moreover, the SUMO conjugating enzyme, Ubc9, is required for RPL11-mediated activation of p53. SUMOylation of RPL11 is triggered by ribosomal stress as well as by ARF. The ribosomal protein L23 and ARF can be also modulated by SUMO. In addition, this study has led us to advance in the knowledge of post-translational modifications by ubiquitin-like proteins demonstrating that SUMO can bind to a substrate in lysine independent manner and that there is an interplay between SUMOylation and NEDDylation.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectRPL11
dc.subjectSUMO
dc.subjectp53
dc.subject.classificationMaterias::Investigación::24 Ciencias de la vida::2415 Biología molecular::241501 Biología molecular de microorganismos
dc.subject.classificationMaterias::Investigación::24 Ciencias de la vida::2407 Biología celular::240701 Cultivo celular
dc.titleRegulation of the RP-MDM2-P53 pathway by SUMO
dc.typeinfo:eu-repo/semantics/doctoralThesis
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro Internacional de Estudos de Doutoramento e Avanzados (CIEDUS)
dc.contributor.affiliationUniversidade de Santiago de Compostela. Escola de Doutoramento Internacional en Ciencias da Saúde
dc.contributor.affiliationUniversidade de Santiago de Compostela. Programa de Doutoramento en Medicina Molecular


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