Real-time investigation of dynamic protein crystallization in living cells
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Título: | Real-time investigation of dynamic protein crystallization in living cells |
Autor/a: | Schönherr, R. Klinge, M. Rudolph, J. M. Fita, K. Rehders, D. Lübber, F. Schnnegans, S. Majoul, I. V. Duszenko, M. Betzel, C. Brandariz Núñez, Alberto Martínez Costas, José Manuel Duden, R. Redecke, L. |
Centro/Departamento: | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares Universidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular |
Data: | 2015 |
Editor: | AIP Publishing |
Cita bibliográfica: | Schönherr, R., Klinge, M., Rudolph, J., Fita, K., Rehders, D., & Lübber, F. et al. (2015). Real-time investigation of dynamic protein crystallization in living cells. Structural Dynamics, 2(4), 041712. doi: 10.1063/1.4921591 |
Resumo: | X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro |
Versión do editor: | https://doi.org/10.1063/1.4921591 |
URI: | http://hdl.handle.net/10347/18330 |
DOI: | 10.1063/1.4921591 |
E-ISSN: | 2329-7778 |
Dereitos: | © Author(s) 2015. This is an open access article published under Creative Commons License Attribution (CC BY) Atribución 4.0 Internacional |
Coleccións
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- BBM-Artigos [91]
- CIQUS-Artigos [474]
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