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dc.contributor.authorSánchez López, Mateo Isidro
dc.contributor.authorPenas Tallón, Cristina
dc.contributor.authorVázquez Sentís, Marco Eugenio
dc.contributor.authorMascareñas Cid, José Luis
dc.identifier.citationSánchez, M., Penas, C., Vázquez, M., & Mascareñas, J. (2014). Metal-catalyzed uncaging of DNA-binding agents in living cells. Chem. Sci., 5(5), 1901-1907. doi: 10.1039/c3sc53317d
dc.description.abstractAttachment of alloc protecting groups to the amidine units of fluorogenic DNA-binding bisbenzamidines or to the amino groups of ethidium bromide leads to a significant reduction of their DNA affinity. More importantly, the active DNA-binding species can be readily regenerated by treatment with ruthenium catalysts in aqueous conditions, even in cell cultures. The catalytic chemical uncaging can be easily monitored by fluorescence microscopy, because the protected products display both different emission properties and cell distribution to the parent compounds
dc.description.sponsorshipWe are thankful for the support given by the Spanish grants SAF2010-20822-C02, CTQ2012-31341, Consolider Ingenio 2010 CSD2007-00006, the Xunta de Galicia, GRC2010/12, GRC2013-041, PGIDIT08CSA-047209PR, the ERDF and the European Research Council (Advanced Grant Na 340055). M. I. S. and C. P. thank the Spanish Ministry for their PhD fellowships
dc.publisherRoyal Society of Chemistry
dc.rights© The Royal Society of Chemistry 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited
dc.titleMetal-catalyzed uncaging of DNA-binding agents in living cells
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica

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