Show simple item record

dc.contributor.authorSánchez López, Mateo Isidro
dc.contributor.authorPenas Tallón, Cristina
dc.contributor.authorVázquez Sentís, Marco Eugenio
dc.contributor.authorMascareñas Cid, José Luis
dc.date.accessioned2019-03-12T07:48:34Z
dc.date.available2019-03-12T07:48:34Z
dc.date.issued2014
dc.identifier.citationSánchez, M., Penas, C., Vázquez, M., & Mascareñas, J. (2014). Metal-catalyzed uncaging of DNA-binding agents in living cells. Chem. Sci., 5(5), 1901-1907. doi: 10.1039/c3sc53317d
dc.identifier.urihttp://hdl.handle.net/10347/18336
dc.description.abstractAttachment of alloc protecting groups to the amidine units of fluorogenic DNA-binding bisbenzamidines or to the amino groups of ethidium bromide leads to a significant reduction of their DNA affinity. More importantly, the active DNA-binding species can be readily regenerated by treatment with ruthenium catalysts in aqueous conditions, even in cell cultures. The catalytic chemical uncaging can be easily monitored by fluorescence microscopy, because the protected products display both different emission properties and cell distribution to the parent compounds
dc.description.sponsorshipWe are thankful for the support given by the Spanish grants SAF2010-20822-C02, CTQ2012-31341, Consolider Ingenio 2010 CSD2007-00006, the Xunta de Galicia, GRC2010/12, GRC2013-041, PGIDIT08CSA-047209PR, the ERDF and the European Research Council (Advanced Grant Na 340055). M. I. S. and C. P. thank the Spanish Ministry for their PhD fellowships
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/340055
dc.rights© The Royal Society of Chemistry 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited
dc.titleMetal-catalyzed uncaging of DNA-binding agents in living cells
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1039/C3SC53317D
dc.relation.publisherversionhttps://doi.org/10.1039/C3SC53317D
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn2041-6539
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.description.peerreviewedSI


Files in this item

application/pdf
Name: 2014_chemsci_sanchez_metal.pdf
Size: 693.1 Kb
Format: PDF


Thumbnail

This item appears in the following Collection(s)

Show simple item record






Harvesters:Useful links:
Universidade de Santiago de Compostela | Teléfonos: +34 881 811 000 e +34 982 820 000 | Contact Us | Send Feedback