A Folding-Based Approach for the Luminescent Detection of a Short RNA Hairpin
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Título: | A Folding-Based Approach for the Luminescent Detection of a Short RNA Hairpin |
Autor/a: | Penas Tallón, Cristina Pazos Chantrero, Elena Mascareñas Cid, José Luis Vázquez Sentís, Marco Eugenio |
Centro/Departamento: | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares Universidade de Santiago de Compostela. Departamento de Química Orgánica |
Data: | 2013 |
Editor: | American Chemical Society |
Cita bibliográfica: | Penas, C., Pazos, E., Mascareñas, J., & Vázquez, M. (2013). A Folding-Based Approach for the Luminescent Detection of a Short RNA Hairpin. J. Am. Chem. Soc., 135, 10, 3812–3814. doi: 10.1021/ja400270a |
Resumo: | We report the rational design of a 20-mer basic peptide, derived from the transcriptional antitermination protein N of bacteriophage P22, equipped with a luminescent DOTA[Tb3+] macrocyclic complex and a sensitizing tryptophan antenna. Folding of this peptide into an α helical conformation, which occurs upon binding to its target boxB RNA hairpin, results in a large increase in luminescence emission. Therefore, the peptide construct works as a highly sensitive and selective probe for this RNA hairpin |
Descrición: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://pubs.acs.org/doi/10.1021/ja400270a |
Versión do editor: | https://doi.org/10.1021/ja400270a |
URI: | http://hdl.handle.net/10347/18479 |
DOI: | 10.1021/ja400270a |
ISSN: | 0002-7863 |
E-ISSN: | 1520-5126 |
Dereitos: | © 2013 American Chemical Society |
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