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dc.contributor.authorFolgueira López, Iria
dc.contributor.authorLamas Fernández, Jesús
dc.contributor.authorFelipe González, Ana Paula de
dc.contributor.authorSueiro Benavides, Rosa Ana
dc.contributor.authorLeiro Vidal, José Manuel
dc.date.accessioned2019-08-21T08:57:21Z
dc.date.available2020-07-05T01:00:09Z
dc.date.issued2019
dc.identifier.citationFolgueira I, Lamas J, De Felipe AP, Sueiro RA, Leiro JM. (2019). Evidence for the role of extrusomes in evading attack by the host immune system in a scuticociliate parasite. Fish Shellfish Immunol. 2019 Jul 5;92:802-812. doi: 10.1016/j.fsi.2019.07.008
dc.identifier.issn1050-4648
dc.identifier.urihttp://hdl.handle.net/10347/19714
dc.descriptionThis is the accepted manuscript of the following article: Folgueira I, Lamas J, De Felipe AP, Sueiro RA, Leiro JM. (2019). Evidence for the role of extrusomes in evading attack by the host immune system in a scuticociliate parasite. Fish Shellfish Immunol. 2019 Jul 5;92:802-812. doi: 10.1016/j.fsi.2019.07.008
dc.description.abstractLike other ciliates, Philasterides dicentrarchi, the scuticociliate parasite of turbot, produces a feeding-only or growing stage called a trophont during its life cycle. Exposure of the trophonts to heat-inactivated serum extracted from the turbot host and containing specific antibodies that induce agglutination/immobilization leads to the production of a mucoid capsule from which the trophonts later emerge. We investigated how these capsules are generated, observing that the mechanism was associated with the process of exocytosis involved in the release of a matrix material from the extrusomes. The extruded material contains mucin-like glycoproteins that were deposited on the surface of the cell and whose expression increased with time of exposure to the heat-inactivated immune serum, at both protein expression and gene expression levels. Stimulation of the trophonts with the immune serum also caused an increase in discharge of the intracellular storage compartments of calcium necessary for the exocytosis processes in the extrusomes. The results obtained suggest that P. dicentrarchi uses the extrusion mechanism to generate a physical barrier protecting the ciliate from attack by soluble factors of the host immune system. Data on the proteins involved and the potential development of molecules that interfere with this exocytic process could contribute to improving the prevention and control of scuticociliatosis in turbot
dc.description.sponsorshipThis study was financially supported by grants from the Ministerio de Economía y Competitividad and Fondo Europeo de Desarrollo Regional -FEDER- (European Union) (AGL2017-83577-R) and from the Xunta de Galicia (ED431C2017/31) and also by the PARAFISHCONTROL project, which received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No. 634429
dc.language.isoeng
dc.publisherElsevier
dc.rights© 2019 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http:// creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPhilasterides dicentrarchi
dc.subjectTurbot
dc.subjectExocytosis
dc.subjectExtrusomes
dc.subjectTrichocyst matrix proteins
dc.subjectMucin-like glycoproteins
dc.titleEvidence for the role of extrusomes in evading attack by the host immune system in a scuticociliate parasite
dc.typejournal article
dc.identifier.doi10.1016/j.fsi.2019.07.008
dc.relation.publisherversionhttps://doi.org/10.1016/j.fsi.2019.07.008
dc.type.hasVersionAM
dc.rights.accessRightsopen access
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioloxía Funcional
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Acuicultura
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Investigación e Análises Alimentarias
dc.description.peerreviewedSI
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/634429
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-83577-R/ES/GENERACION DE VACUNAS RECOMBINANTES PARA LA INMUNOPROFILAXIS DE LA ESCUTICOCILIATOSIS DEL RODABALLO


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© 2019 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http:// creativecommons.org/licenses/by-nc-nd/4.0/)
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 © 2019 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http:// creativecommons.org/licenses/by-nc-nd/4.0/)





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