Investigating Possible Enzymatic Degradation on Polymer Shells around Inorganic Nanoparticles
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Título: | Investigating Possible Enzymatic Degradation on Polymer Shells around Inorganic Nanoparticles |
Autor/a: | Zhu, Lin Pelaz García, Beatriz Chakraborty, Indranath Parak, Wolfgang J. |
Centro/Departamento: | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares Universidade de Santiago de Compostela. Departamento de Física de Partículas |
Palabras chave: | Nanoparticles | Surface engineering | Enzymatic degradation | Polymer coating | Bioconjugation | Click chemistry | Nanoparticle degradation | |
Data: | 2019 |
Editor: | Universidade de Santiago de Compostela |
Cita bibliográfica: | Zhu, L.; Pelaz, B.; Chakraborty, I.; Parak, W.J. Investigating Possible Enzymatic Degradation on Polymer Shells around Inorganic Nanoparticles. Int. J. Mol. Sci. 2019, 20, 935 |
Resumo: | Inorganic iron oxide nanoparticle cores as model systems for inorganic nanoparticles were coated with shells of amphiphilic polymers, to which organic fluorophores were linked with different conjugation chemistries, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry and two types of “click chemistry”. The nanoparticle-dye conjugates were exposed to different enzymes/enzyme mixtures in order to investigate potential enzymatic degradation of the fluorophore-modified polymer shell. The release of the dyes and polymer fragments upon enzymatic digestion was quantified by using fluorescence spectroscopy. The data indicate that enzymatic cleavage of the fluorophore-modified organic surface coating around the inorganic nanoparticles in fact depends on the used conjugation chemistry, together with the types of enzymes to which the nanoparticle-dye conjugates are exposed |
Versión do editor: | https://doi.org/10.3390/ijms20040935 |
URI: | http://hdl.handle.net/10347/19756 |
DOI: | 10.3390/ijms20040935 |
ISSN: | 1661-6596 |
E-ISSN: | 1422-0067 |
Dereitos: | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) Atribución 4.0 Internacional |
Coleccións
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- CIQUS-Artigos [475]
- FP-Artigos [462]
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