In situ detection of the protein corona in complex environments
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| Title: | In situ detection of the protein corona in complex environments |
| Author: | Carril, Mónica Prado, Daniel Pino, Pablo del Carrillo Carrión, Carolina Gallego, Marta Parak, Wolfgang J. |
| Affiliation: | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares Universidade de Santiago de Compostela. Departamento de Física de Partículas |
| Subject: | Biophysical chemistry | Nanoparticles | NMR spectroscopy | Surface chemistry | |
| Date of Issue: | 2017 |
| Publisher: | Springer Nature |
| Citation: | Carril, M., Padro, D., del Pino, P., Carrillo-Carrion, C., Gallego, M., & Parak, W. (2017). In situ detection of the protein corona in complex environments. Nature Communications, 8(1). doi: 10.1038/s41467-017-01826-4 |
| Abstract: | Colloidal nanoparticles (NPs) are a versatile potential platform for in vivo nanomedicine. Inside blood circulation, NPs may undergo drastic changes, such as by formation of a protein corona. The in vivo corona cannot be completely emulated by the corona formed in blood. Thus, in situ detection in complex media, and ultimately in vivo, is required. Here we present a methodology for determining protein corona formation in complex media. NPs are labeled with 19F and their diffusion coefficient measured using 19F diffusion-ordered nuclear magnetic resonance (NMR) spectroscopy. 19F diffusion NMR measurements of hydrodynamic radii allow for in situ characterization of NPs in complex environments by quantification of protein adsorption to the surface of NPs, as determined by increase in hydrodynamic radius. The methodology is not optics based, and thus can be used in turbid environments, as in the presence of cells |
| Publisher version: | https://doi.org/10.1038/s41467-017-01826-4 |
| URI: | http://hdl.handle.net/10347/19758 |
| DOI: | 10.1038/s41467-017-01826-4 |
| E-ISSN: | 2041-1723 |
| Rights: | © The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional |
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Except where otherwise noted, this item's license is described as © The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/