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dc.contributor.authorPérez-Hernández, Marta
dc.contributor.authorMoros, María
dc.contributor.authorStepien, Grazyna
dc.contributor.authorPino, Pablo del
dc.contributor.authorMenao, Sebastián
dc.contributor.authorHeras, Marcelo de las
dc.contributor.authorArias, Maykel
dc.contributor.authorMitchell, Scott G.
dc.contributor.authorPelaz García, Beatriz
dc.contributor.authorGálvez, Eva M.
dc.contributor.authorMartínez de la Fuente, Jesús
dc.contributor.authorPardo, Julián
dc.date.accessioned2019-09-11T11:22:17Z
dc.date.available2019-09-11T11:22:17Z
dc.date.issued2017
dc.identifier.citationPérez-Hernández, M., Moros, M., Stepien, G., del Pino, P., Menao, S., & de las Heras, M. et al. (2017). Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo. Particle And Fibre Toxicology, 14(1). doi: 10.1186/s12989-017-0222-4
dc.identifier.issn1743-8977
dc.identifier.urihttp://hdl.handle.net/10347/19762
dc.description.abstractBackground The special physicochemical properties of gold nanoprisms make them very useful for biomedical applications including biosensing and cancer therapy. However, it is not clear how gold nanoprisms may affect cellular physiology including viability and other critical functions. We report a multiparametric investigation on the impact of gold-nanoprisms on mice and human, transformed and primary cells as well as tissue distribution and toxicity in vivo after parental injection. Methods Cellular uptake of the gold-nanoprisms (NPRs) and the most crucial parameters of cell fitness such as generation of reactive oxygen species (ROS), mitochondria membrane potential, cell morphology and apoptosis were systematically assayed in cells. Organ distribution and toxicity including inflammatory response were analysed in vivo in mice at 3 days or 4 months after parental administration. Results Internalized gold-nanoprisms have a significant impact in cell morphology, mitochondrial function and ROS production, which however do not affect the potential of cells to proliferate and form colonies. In vivo NPRs were only detected in spleen and liver at 3 days and 4 months after administration, which correlated with some changes in tissue architecture. However, the main serum biochemical markers of organ damage and inflammation (TNFα and IFNγ) remained unaltered even after 4 months. In addition, animals did not show any macroscopic sign of toxicity and remained healthy during all the study period. Conclusion Our data indicate that these gold-nanoprisms are neither cytotoxic nor cytostatic in transformed and primary cells, and suggest that extensive parameters should be analysed in different cell types to draw useful conclusions on nanomaterials safety. Moreover, although there is a tendency for the NPRs to accumulate in liver and spleen, there is no observable negative impact on animal health
dc.description.sponsorshipThis work was supported by the MAT2011–26851-CO2–01, MAT2011–26851-CO2–02, SAF2011–25390, SAF2014–54763-C2–1-R and SAF2014–54763-C2–2-R - projects of the Spanish Ministry of Science and Innovation, PIE201460I019 (CSIC), the grant ERC-Starting Grant 239931-NANOPUZZLE project, ERC-PoC 754609 HOTFLOW project, Fondo Social Europeo (FSE; Gobierno de Aragón) and the Consellería de Cultura, Educación e Ordenación Universitaria (Centro singular de investigación de Galicia accreditation 2016–2019, ED431G/09) and the European Regional Development Fund (ERDF). P.d.P., J.M.F., J.P. thank ARAID for financial support, B.P. thanks the Alexander von Humboldt Foundation for a fellowship and M.M. acknowledges financial support from European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 660228
dc.language.isoeng
dc.publisherBioMed Central
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/239931
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/754609
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/660228
dc.rights© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectGold nanoprisms
dc.subjectNanotoxicology
dc.subjectROS generation
dc.subjectMitochondrial membrane-potential
dc.subjectBiodistribution
dc.subjectIn vivo
dc.titleMultiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1186/s12989-017-0222-4
dc.relation.publisherversionhttps://doi.org/10.1186/s12989-017-0222-4
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Física de Partículas
dc.description.peerreviewedSI


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© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
Except where otherwise noted, this item's license is described as  © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated





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