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dc.contributor.authorBarbosa, Mélanie Audrey Gomes
dc.contributor.authorCapela, Ricardo
dc.contributor.authorRodolfo, Jorge
dc.contributor.authorFonseca, Elza
dc.contributor.authorAndré, Ana
dc.contributor.authorCapitão, Ana
dc.contributor.authorCarvalho, António Paulo
dc.contributor.authorQuintana Álvarez, José Benito
dc.contributor.authorCastro, L. Filipe C.
dc.contributor.authorSantos, Miguel Machado
dc.date.accessioned2020-01-22T14:11:14Z
dc.date.available2021-07-06T01:00:09Z
dc.date.issued2019-07-06
dc.identifier.citationBarbosa et al. Linking chemical exposure to lipid homeostasis: A municipal waste water treatment plant influent is obesogenic for zebrafish larvae. Ecotoxicology and Environmental Safety, 2019, 182, 109406
dc.identifier.issn0147-6513
dc.identifier.urihttp://hdl.handle.net/10347/20605
dc.description.abstractObesity, a risk factor for the development of type-2 diabetes, hypertension, cardiovascular disease, hepatic steatosis and some cancers, has been ranked in the top 10 health risk in the world by the World Health Organization. Despite the growing body of literature evidencing an association between the obesity epidemic and specific chemical exposure across a wide range of animal taxa, very few studies assessed the effects of chemical mixtures and environmental samples on lipid homeostasis. Additionally, the mode of action of several chemicals reported to alter lipid homeostasis is still poorly understood. Aiming to fill some of these gaps, we combined an in vivo assay with the model species zebrafish (Danio rerio) to screen lipid accumulation and evaluate expression changes of key genes involved in lipid homeostasis, alongside with an in vitro transactivation assay using human and zebrafish nuclear receptors, retinoid X receptor α and peroxisome proliferator-activated receptor γ. Zebrafish larvae were exposed from 4 th day post-fertilization until the end of the experiment (day 18), to six different treatments: experimental control, solvent control, tributyltin at 100 ng/L Sn and 200 ng/L Sn (positive control), and wastewater treatment plant influent at 1.25% and 2.5%. Exposure to tributyltin and to 2.5% influent led to a significant accumulation of lipids, with white adipose tissue deposits concentrating in the perivisceral area. The highest in vitro tested influent concentration (10%) was able to significantly transactivate the human heterodimer PPARγ/RXRα, thus suggesting the presence in the influent of HsPPARγ/RXRα agonists. Our results demonstrate, for the first time, the ability of complex environmental samples from a municipal waste water treatment plant influent to induce lipid accumulation in zebrafish larvae.
dc.description.sponsorshipThis work was supported by the Norte2020 and FEDER (Coral—Sustainable Ocean Exploitation—Norte-01-0145-FEDER-000036), by the Spanish Agencia Estatal de Investigación (CTM2017-84763-C3-2-R), the Galician Council of Culture, Education and Universities (ED431C2017/36) and FEDER/ERDF, and by the project NOR-WATER (0725), financed by “Programa de Cooperação Interreg Portugal/Espanha, (POCTEP) 2014-2020. Ricardo Capela was supported by grant SFRH/BD/112483/2015
dc.language.isoeng
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTM2017-84763-C3-2-R/ES/EVALUACION DEL EXPOSOMA DE CONTAMINANTES EMERGENTES EN AMBIENTES ACUATICOS
dc.rights© 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectObesity
dc.subjectObesogen
dc.subjectEndocrine disruptor
dc.subjectEnvironmental mixture
dc.subjectNuclear receptor
dc.titleLinking chemical exposure to lipid homeostasis: A municipal waste water treatment plant influent is obesogenic for zebrafish larvae
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1016/j.ecoenv.2019.109406
dc.relation.publisherversionhttps://doi.org/10.1016/j.ecoenv.2019.109406
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Investigación e Análises Alimentarias
dc.description.peerreviewedSI


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© 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Except where otherwise noted, this item's license is described as  © 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)





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