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dc.contributor.author | Núñez Iglesias, María Jesús |
dc.contributor.author | Novío Mallón, Silvia |
dc.contributor.author | García Santiago, Carlota |
dc.contributor.author | Pérez Muñuzuri, Elena |
dc.contributor.author | Soengas Fernández, María del Pilar |
dc.contributor.author | Cartea González, María Elena |
dc.contributor.author | Velasco Pazos, Pablo |
dc.contributor.author | Freire-Garabal Núñez, Manuel |
dc.date.accessioned | 2020-04-15T12:53:58Z |
dc.date.available | 2020-04-15T12:53:58Z |
dc.date.issued | 2019 |
dc.identifier.citation | Núñez-Iglesias, M. J., Novío, S., García, C., Pérez-Muñuzuri, E., Soengas, P., Cartea, E., . . . Freire-Garabal, M. (2019). Glucosinolate-degradation products as co-adjuvant therapy on prostate cancer in vitro. International Journal of Molecular Sciences, 20(20), 4977. doi:10.3390/ijms20204977 |
dc.identifier.uri | http://hdl.handle.net/10347/21414 |
dc.description.abstract | Glucosinolate-degradation products (GS-degradation products) are believed to be responsible for the anticancer effects of cruciferous vegetables. Furthermore, they could improve the efficacy and reduce side-effects of chemotherapy. The aim of the present study was to determine the cytotoxic effects of GS-degradation products on androgen-insensitive human prostate cancer (AIPC) PC-3 and DU 145 cells and investigate their ability to sensitize such cells to chemotherapeutic drug Docetaxel (DOCE). Cells were cultured under growing concentrations of allyl-isothiocyanate (AITC), sulforaphane (SFN), 4-pentenyl-isothiocyanate (4PI), iberin (IB), indole-3-carbinol (I3C), or phenethyl-isothiocyanate (PEITC) in absence or presence of DOCE. The anti-tumor effects of these compounds were analyzed using the trypan blue exclusion, apoptosis, invasion and RT-qPCR assays and confocal microscopy. We observed that AITC, SFN, IB, and/or PEITC induced a dose- and time-dependent cytotoxic effect on PC-3 and DU 145 cells, which was mediated, at least, by apoptosis and cell cycle arrest. Likewise, we showed that these GS-degradation products sensitized both cell lines to DOCE by synergic mechanisms. Taken together, our results indicate that GS-degradation products can be promising compounds as co-adjuvant therapy in prostate cancer. |
dc.description.sponsorship | This research was supported by the Spanish National Plan for Research and Development, grant number AGL2012-9 35539, and financed by the European Regional Development Funds (FEDER) |
dc.language.iso | eng |
dc.publisher | MDPI |
dc.rights | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ |
dc.subject | Chemoprevention |
dc.subject | Docetaxel |
dc.subject | Drug-sensitization |
dc.subject | Isothiocyanates |
dc.subject | Prostate cancer |
dc.subject | Synergism |
dc.title | Glucosinolate-Degradation Products as Co-Adjuvant Therapy on Prostate Cancer in Vitro |
dc.type | journal article |
dc.identifier.doi | 10.3390/ijms20204977 |
dc.relation.publisherversion | https://doi.org/10.3390/ijms20204977 |
dc.type.hasVersion | VoR |
dc.identifier.essn | 1422-0067 |
dc.rights.accessRights | open access |
dc.contributor.affiliation | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
dc.contributor.affiliation | Universidade de Santiago de Compostela. Departamento de Psiquiatría, Radioloxía, Saúde Pública, Enfermaría e Medicina |
dc.description.peerreviewed | SI |
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