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dc.contributor.authorNúñez Iglesias, María Jesús
dc.contributor.authorNovío Mallón, Silvia
dc.contributor.authorGarcía Santiago, Carlota
dc.contributor.authorPérez Muñuzuri, Elena
dc.contributor.authorSoengas Fernández, María del Pilar
dc.contributor.authorCartea González, María Elena
dc.contributor.authorVelasco Pazos, Pablo
dc.contributor.authorFreire-Garabal Núñez, Manuel
dc.date.accessioned2020-04-15T12:53:58Z
dc.date.available2020-04-15T12:53:58Z
dc.date.issued2019
dc.identifier.citationNúñez-Iglesias, M. J., Novío, S., García, C., Pérez-Muñuzuri, E., Soengas, P., Cartea, E., . . . Freire-Garabal, M. (2019). Glucosinolate-degradation products as co-adjuvant therapy on prostate cancer in vitro. International Journal of Molecular Sciences, 20(20), 4977. doi:10.3390/ijms20204977
dc.identifier.urihttp://hdl.handle.net/10347/21414
dc.description.abstractGlucosinolate-degradation products (GS-degradation products) are believed to be responsible for the anticancer effects of cruciferous vegetables. Furthermore, they could improve the efficacy and reduce side-effects of chemotherapy. The aim of the present study was to determine the cytotoxic effects of GS-degradation products on androgen-insensitive human prostate cancer (AIPC) PC-3 and DU 145 cells and investigate their ability to sensitize such cells to chemotherapeutic drug Docetaxel (DOCE). Cells were cultured under growing concentrations of allyl-isothiocyanate (AITC), sulforaphane (SFN), 4-pentenyl-isothiocyanate (4PI), iberin (IB), indole-3-carbinol (I3C), or phenethyl-isothiocyanate (PEITC) in absence or presence of DOCE. The anti-tumor effects of these compounds were analyzed using the trypan blue exclusion, apoptosis, invasion and RT-qPCR assays and confocal microscopy. We observed that AITC, SFN, IB, and/or PEITC induced a dose- and time-dependent cytotoxic effect on PC-3 and DU 145 cells, which was mediated, at least, by apoptosis and cell cycle arrest. Likewise, we showed that these GS-degradation products sensitized both cell lines to DOCE by synergic mechanisms. Taken together, our results indicate that GS-degradation products can be promising compounds as co-adjuvant therapy in prostate cancer.
dc.description.sponsorshipThis research was supported by the Spanish National Plan for Research and Development, grant number AGL2012-9 35539, and financed by the European Regional Development Funds (FEDER)
dc.language.isoeng
dc.publisherMDPI
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectChemoprevention
dc.subjectDocetaxel
dc.subjectDrug-sensitization
dc.subjectIsothiocyanates
dc.subjectProstate cancer
dc.subjectSynergism
dc.titleGlucosinolate-Degradation Products as Co-Adjuvant Therapy on Prostate Cancer in Vitro
dc.typejournal article
dc.identifier.doi10.3390/ijms20204977
dc.relation.publisherversionhttps://doi.org/10.3390/ijms20204977
dc.type.hasVersionVoR
dc.identifier.essn1422-0067
dc.rights.accessRightsopen access
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Psiquiatría, Radioloxía, Saúde Pública, Enfermaría e Medicina
dc.description.peerreviewedSI


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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
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 © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)





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