| dc.contributor.author | Iglesias, Pablo |
| dc.contributor.author | Penas Tallón, Cristina |
| dc.contributor.author | Barral Cagiao, Laura |
| dc.contributor.author | Pazos Chantrero, Elena |
| dc.contributor.author | Costoya Puente, José Antonio |
| dc.date.accessioned | 2020-04-20T14:25:20Z |
| dc.date.available | 2020-04-20T14:25:20Z |
| dc.date.issued | 2019 |
| dc.identifier.citation | Iglesias, P., Penas, C., Barral-Cagiao, L. et al. (2019). A Bio-inspired Hypoxia Sensor using HIF1a-Oxygen-Dependent Degradation Domain. Sci Rep 9, 7117. doi:10.1038/s41598-019-43618-4 |
| dc.identifier.uri | http://hdl.handle.net/10347/21556 |
| dc.description.abstract | Functional imaging has become an important tool in oncology because it not only provides information
about the size and localization of the tumour, but also about the pathophysiological features of the
tumoural cells. One of the characteristic features of some tumour types is that their fast growth leads
to defcient intratumoral vascularization, which results in low oxygen availability. To overcome this
lack of oxygen, tumoural cells activate the neoangiogenic program by upregulating the transcription
factor HIF-1α. Herein we report a non-invasive in vitro detection method of hypoxia using designed
fuorescent peptide probes based on the oxygen-dependent degradation domain of HIF-1α. The
fuorescent probe retains the oxygen-sensing capability of HIF-1α, so that it is stabilized under hypoxia
and readily degraded by the proteasome under normoxia, thus providing direct information of the
cellular oxygen availability |
| dc.description.sponsorship | This work has received financial support from the Spanish MICINN (SAF2009-08629; J.A.C.) and ISCIII, MINECO (PI15/01129), AEI/FEDER, UE (CTQ2017-89166-R; E.P.), the Consellería de Cultura, Educación e Ordenación Universitaria (GPC2014/030, INCITE08PXIB208091PR; J.A.C.), the Xunta de Galicia (Centro Singular de Investigación de Galicia accreditations 2016‐2019, ED431G/05 and ED431G/09) and the European Regional Development Fund (ERDF). C.P. thanks the Spanish MICINN for her PhD fellowship. E.P. thanks the UDC-Inditex InTalent Programme for her research contract and funding |
| dc.language.iso | eng |
| dc.publisher | Nature Publishing Group |
| dc.relation | info:eu-repo/grantAgreement/MICINN/Plan Nacional de I+D+i 2008-2011/SAF2009-08629/ES/Caracterizacion Molecular De Los Mecanismos Implicados En La Transicion De Gliomas De Bajo A Alto Grado |
| dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-89166-R/ES/HERRAMIENTAS PEPTIDICAS PARA APLICACIONES BIOLOGICAS: SENSORES INTELIGENTES PARA ESTUDIAR NITRACION DE PROTEINAS Y NANOMATERIALES CON FUNCIONALIDADES MODULABLES |
| dc.rights | © The Author(s) 2019. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ |
| dc.subject | Chemical biology |
| dc.subject | Cancer |
| dc.title | A bio-inspired hypoxia sensor using HIF1a-oxygen-dependent degradation domain |
| dc.type | info:eu-repo/semantics/article |
| dc.identifier.DOI | 10.1038/s41598-019-43618-4 |
| dc.relation.publisherversion | https://doi.org/10.1038/s41598-019-43618-4 |
| dc.type.version | info:eu-repo/semantics/publishedVersion |
| dc.identifier.e-issn | 2045-2322 |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess |
| dc.contributor.affiliation | Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas |
| dc.contributor.affiliation | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares |
| dc.contributor.affiliation | Universidade de Santiago de Compostela. Departamento de Fisioloxía |
| dc.contributor.affiliation | Universidade de Santiago de Compostela. Departamento de Química Orgánica |
| dc.description.peerreviewed | SI |
