Computer-Assisted Recovery of Threatened Plants: Keys for Breaking Seed Dormancy of Eryngium viviparum
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Title: | Computer-Assisted Recovery of Threatened Plants: Keys for Breaking Seed Dormancy of Eryngium viviparum |
Author: | Ayuso, Manuel Ramil Rego, Pablo Landín Pérez, Mariana Gallego, Pedro P. Barreal, M. Esther |
Affiliation: | Universidade de Santiago de Compostela. Departamento de Botánica Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica Universidade de Santiago de Compostela. Instituto de Biodiversidade Agraria e Desenvolvemento Rural (IBADER) |
Subject: | Apiaceae | Artificial intelligence | Conservation | Germination | Dormancy | Endemic plant | Embryo seed ratio | Underdeveloped embryo | |
Date of Issue: | 2017 |
Publisher: | Frontiers Media |
Citation: | Ayuso M, Ramil-Rego P, Landin M, Gallego PP and Barreal ME (2017) Computer-Assisted Recovery of Threatened Plants: Keys for Breaking Seed Dormancy of Eryngium viviparum. Front. Plant Sci. 8:2092. doi: 10.3389/fpls.2017.02092 |
Abstract: | Many endangered plants such as Eryngium viviparum (Apiaceae) present a poor germination rate. This fact could be due to intrinsic and extrinsic seed variability influencing germination and dormancy of seeds. The objective of this study is to better understand the physiological mechanism of seed latency and, through artificial intelligence models, to determine the factors that stimulate germination rates of E. viviparum seeds. This description could be essential to prevent the disappearance of endangered plants. Germination in vitro was carried out under different dormancy breaking and incubation procedures. Percentages of germination, viability and E:S ratio were calculated and seeds were dissected at the end of each assay to describe embryo development. The database obtained was modeled using neurofuzzy logic technology. We have found that the most of Eryngium seeds (62.6%) were non-viable seeds (fully empty or without embryos). Excluding those, we have established the germination conditions to break seed dormancy that allow obtaining a real germination rate of 100%. Advantageously, the best conditions pointed out by neurofuzzy logic model for embryo growth were the combination of 1 mg L−1 GA3 (Gibberellic Acid) and high incubation temperature and for germination the combination of long incubation and short warm stratification periods. Our results suggest that E. viviparum seeds present morphophysiological dormancy, which reduce the rate of germination. The knowledge provided by the neurofuzzy logic model makes possible not just break the physiological component of dormancy, but stimulate the embryo development increasing the rate of germination. Undoubtedly, the strategy developed in this work can be useful to recover other endangered plants by improving their germination rate and uniformity favoring their ex vitro conservation |
Publisher version: | https://doi.org/10.3389/fpls.2017.02092 |
URI: | http://hdl.handle.net/10347/21564 |
DOI: | 10.3389/fpls.2017.02092 |
E-ISSN: | 1664-462X |
Rights: | Copyright © 2017 Ayuso, Ramil-Rego, Landin, Gallego and Barreal. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms Atribución 4.0 Internacional |
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Copyright © 2017 Ayuso, Ramil-Rego, Landin, Gallego and Barreal. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
Copyright © 2017 Ayuso, Ramil-Rego, Landin, Gallego and Barreal. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms