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dc.contributor.authorLi, Hongzhao
dc.contributor.authorNykoluk, Mikaela
dc.contributor.authorLi, Lin
dc.contributor.authorLiu, Lewis R.
dc.contributor.authorOmange, Robert W.
dc.contributor.authorSoule, Geoff
dc.contributor.authorSchroeder, Lukas T.
dc.contributor.authorToledo, Nikki
dc.contributor.authorKashem, Mohammad Abul
dc.contributor.authorCorreia Pinto, Jorge F.
dc.contributor.authorLiang, Binhua
dc.contributor.authorSchultz-Darken, Nancy
dc.contributor.authorAlonso Fernández, María José
dc.contributor.authorWhitney, James B.
dc.contributor.authorPlummer, Francis A.
dc.contributor.authorLuo, Ma
dc.date.accessioned2020-04-21T20:20:20Z
dc.date.available2020-04-21T20:20:20Z
dc.date.issued2017
dc.identifier.citationLi H, Nykoluk M, Li L, Liu LR, Omange RW, Soule G, et al. (2017) Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques. PLoS ONE 12(10): e0186079. https://doi.org/10.1371/journal.pone.0186079
dc.identifier.urihttp://hdl.handle.net/10347/21611
dc.description.abstractCynomolgus macaques are an increasingly important nonhuman primate model for HIV vaccine research. SIV-free animals without pre-existing anti-SIV immune responses are generally needed to evaluate the effect of vaccine-induced immune responses against the vaccine epitopes. Here, in order to select such animals for vaccine studies, we screened 108 naïve female Mauritian cynomolgus macaques for natural (baseline) antibodies to SIV antigens using a Bio-Plex multiplex system. The antigens included twelve 20mer peptides overlapping the twelve SIV protease cleavage sites (-10/+10), respectively (PCS peptides), and three non-PCS Gag or Env peptides. Natural antibodies to SIV antigens were detected in subsets of monkeys. The antibody reactivity to SIV was further confirmed by Western blot using purified recombinant SIV Gag and Env proteins. As expected, the immunization of monkeys with PCS antigens elicited anti-PCS antibodies. However, unexpectedly, antibodies to non-PCS peptides were also induced, as shown by both Bio-Plex and Western blot analyses, while the non-PCS peptides do not share sequence homology with PCS peptides. The presence of natural and vaccine cross-inducible SIV antibodies in Mauritian cynomolgus macaques should be considered in animal selection, experimental design and result interpretation, for their best use in HIV vaccine research
dc.description.sponsorshipThis work was supported by an NIH grant (R01AI111805), a CIHR/CHVI bridging grant and funding from National Microbiology Laboratory of Canada
dc.language.isoeng
dc.publisherPLOS
dc.rights© 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleNatural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1371/journal.pone.0186079
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0186079
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn1932-6203
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
dc.description.peerreviewedSI


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© 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Except where otherwise noted, this item's license is described as  © 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited





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