Show simple item record

dc.contributor.authorRombouts, Julius Laurens
dc.contributor.authorKranendonk, Elsemiek Madeleine Maria
dc.contributor.authorRegueira López, Alberte
dc.contributor.authorWeissbrodt, David Gregory
dc.contributor.authorKleerebezem, Robbert
dc.contributor.authorLoosdrecht, Mark Cornelis Maria van
dc.date.accessioned2020-04-23T15:15:48Z
dc.date.available2020-04-23T15:15:48Z
dc.date.issued2020
dc.identifier.citationRombouts, JL, Kranendonk, EMM, Regueira, A, Weissbrodt, DG, Kleerebezem, R, van Loosdrecht, MCM. Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures. Biotechnology and Bioengineering. 2020; 117: 1281– 1293. https://doi.org/10.1002/bit.27301
dc.identifier.urihttp://hdl.handle.net/10347/21669
dc.description.abstractLactic acid‐producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology‐based process design and can aid the development of sustainable and energy‐efficient bioprocesses. Here we demonstrate the enrichment of lactic acid bacteria in a controlled sequencing batch bioreactor environment using a glucose‐based medium supplemented with peptides and B vitamins. A mineral medium enrichment operated in parallel was dominated by Ethanoligenens species and fermented glucose to acetate, butyrate and hydrogen. The complex medium enrichment was populated by Lactococcus, Lactobacillus and Megasphaera species and showed a product spectrum of acetate, ethanol, propionate, butyrate and valerate. An intermediate peak of lactate was observed, showing the simultaneous production and consumption of lactate, which is of concern for lactic acid production purposes. This study underlines that the competitive advantage for lactic acid‐producing bacteria primarily lies in their ability to attain a high biomass specific uptake rate of glucose, which was two times higher for the complex medium enrichment when compared to the mineral medium enrichment. The competitive advantage of lactic acid production in rich media can be explained using a resource allocation theory for microbial growth processes
dc.description.sponsorshipNederlandse Organisatie voor Wetenschappelijk Onderzoek, Grant/Award Number: 024.002.002; Spanish Ministry of Education, Grant/Award Number: FPU14/05457
dc.language.isoeng
dc.publisherWiley
dc.rights© 2020 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes
dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectEnrichment cultures
dc.subjectKinetics
dc.subjectLactic acid bacteria
dc.subjectMicrobial ecology
dc.subjectResource allocation
dc.titleSelecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1002/bit.27301
dc.relation.publisherversionhttps://doi.org/10.1002/bit.27301
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn1097-0290
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto Interdisciplinar de Tecnoloxías Ambientais (CRETUS)
dc.description.peerreviewedSI


Files in this item

application/pdf
Name: 2020_bit_rombouts_selecting.pdf
Size: 3.131 Mb
Format: PDF


application/pdf
Name: 2020_bit_rombouts_selecting_supp_info.pdf
Size: 775.3 Kb
Format: PDF


Thumbnail

This item appears in the following Collection(s)

Show simple item record

© 2020 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes
This item's license is described as
 © 2020 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes





Harvesters:Interesting links:
Universidade de Santiago de Compostela | Teléfonos: +34 881 811 000 e +34 982 820 000 | Contact Us | Send Feedback