Rare failures of DNA bar codes to separate morphologically distinct species in a biodiversity survey of Iberian leaf beetles
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|Title:||Rare failures of DNA bar codes to separate morphologically distinct species in a biodiversity survey of Iberian leaf beetles
|Author:||Baselga Fraga, Andrés
Gómez Rodríguez, Carola
Novoa Docet, Francisco
Vogler, Alfried P.
|Affiliation:||Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física
|Date of Issue:||2013
|Citation:||Baselga A, Gómez-Rodríguez C, Novoa F, Vogler AP (2013) Rare Failures of DNA Bar Codes to Separate Morphologically Distinct Species in a Biodiversity Survey of Iberian Leaf Beetles. PLoS ONE 8(9): e74854. https://doi.org/10.1371/journal.pone.0074854
|Abstract:||During a survey of genetic and species diversity patterns of leaf beetle (Coleoptera: Chrysomelidae) assemblages across the Iberian Peninsula we found a broad congruence between morphologically delimited species and variation in the cytochrome oxidase (cox1) gene. However, one species pair each in the genera Longitarsus Berthold and Pachybrachis Chevrolat was inseparable using molecular methods, whereas diagnostic morphological characters (including male or female genitalia) unequivocally separated the named species. Parsimony haplotype networks and maximum likelihood trees built from cox1 showed high genetic structure within each species pair, but no correlation with the morphological types and neither with geographic distributions. This contrasted with all analysed congeneric species, which were recovered as monophyletic. A limited number of specimens were sequenced for the nuclear 18S rRNA gene, which showed no or very limited variation within the species pair and no separation of morphological types. These results suggest that processes of lineage sorting for either group are lagging behind the clear morphological and presumably reproductive separation. In the Iberian chrysomelids, incongruence between DNA-based and morphological delimitations is a rare exception, but the discovery of these species pairs may be useful as an evolutionary model for studying the process of speciation in this ecological and geographical setting. In addition, the study of biodiversity patterns based on DNA requires an evolutionary understanding of these incongruences and their potential causes.|
|Rights:||© 2013 Baselga et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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