Virulence patterns in a murine sepsis model of ST131 escherichia coli clinical isolates belonging to serotypes O25b:H4 and O16: H5 are associated to specific virotypes
Por favor, use este identificador para citas ou ligazóns a este ítem:
http://hdl.handle.net/10347/22009
Ficheiros no ítem
Metadatos do ítem
Título: | Virulence patterns in a murine sepsis model of ST131 escherichia coli clinical isolates belonging to serotypes O25b:H4 and O16: H5 are associated to specific virotypes |
Autor/a: | Mora Gutiérrez, Azucena Dahbi, Ghizlane López Capón, Cecilia Mamani Huarani, Rosalía Seferina Marzoa Fandiño, Juan Dion, Sara Picard, Bertrand Blanco Álvarez, Miguel Alonso, María Pilar Denamur, Erick Blanco Álvarez, Jorge |
Centro/Departamento: | Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía |
Palabras chave: | Escherichia coli | Murine Sepsis | |
Data: | 2014 |
Editor: | Plos |
Cita bibliográfica: | Mora Gutiérrez, A., Dahbi, G., López, C., Mamani, R., Marzoa J., Dion, S. et al. (2014). Virulence patterns in a murine sepsis model of ST131 escherichia coli clinical isolates belonging to serotypes O25b:H4 and O16:H5 are associated to specific virotypes. PLoS ONE vol.9(1): e87025 |
Resumo: | Escherichia coli sequence type (ST)131 is an emerging disseminated public health threat implicated in multidrug-resistant extraintestinal infections worldwide. Although the majority of ST131 isolates belong to O25b:H4 serotype, new variants with different serotypes, STs using the discriminative multilocus sequence typing scheme of Pasteur Institute, and virulence-gene profiles (virotypes) have been reported with unknown implications on the pattern of spread, persistence and virulence. The aim of the present study was to compare virulence in a mouse subcutaneous sepsis model of representative ST131 clinical isolates belonging to 2 serotypes (O25b:H4, O16:H5) and nine virotypes and subtypes (A, B, C, D1, D2, D3, D4, D5 and E). Fourteen out of the 23 ST131 isolates tested (61%) killed 90 to 100% of mice challenged, and 18 of 23 (78%) at least 50%. Interestingly, different virulence patterns in association with virotypes were observed, from highly rapid lethality (death in less than 24 h) to low final lethality (death at 7 days) but with presence of an acute inflammation. This is the first study to assess virulence of ST131 isolates belonging to serotype O16:H5, which exhibited virotype C. In spite of their low virulencegene score, O16:H5 isolates did not show significant differences in final lethality compared with highly virulent O25b:H4 isolates of virotypes A, B and C, but killed mice less rapidly. Significant differences were found, however, between virotypes A, B, C (final lethality $80% of mice challenged) and virotypes D, E. Particularly unexpected was the low lethality of the newly assigned virotype E taking into account that it exhibited high virulence-gene score, and the same clonotype H30 as highly virulent O25b:H4 isolates of virotypes A, B and C. In vivo virulence diversity reported in this study would reflect the genetic variability within ST131 clonal group evidenced by molecular typing |
Versión do editor: | https://doi.org/10.1371/journal.pone.0087025 |
URI: | http://hdl.handle.net/10347/22009 |
DOI: | 10.1371/journal.pone.0087025 |
E-ISSN: | 1932-6203 |
Dereitos: | © 2014 Mora et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
Coleccións
-
- MP-Artigos [159]