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dc.contributor.authorSchonherr, R.
dc.contributor.authorKlinge, M.
dc.contributor.authorRudolph, J. M.
dc.contributor.authorFita, K.
dc.contributor.authorRehders, D.
dc.contributor.authorLubber, F.
dc.contributor.authorSchneegans, S.
dc.contributor.authorMajoul, I. V.
dc.contributor.authorDuszenko, M.
dc.contributor.authorBetzel, C.
dc.contributor.authorBrandariz Núñez, Alberto
dc.contributor.authorMartínez Costas, José Manuel
dc.contributor.authorDuden, R.
dc.contributor.authorRedecke, L.
dc.identifier.citationSchonherr, R., Klinge, M., Rudolph, J.M. et al. (2015). Real-time investigation of dynamic protein crystallization in living cells, "Structural Dynamics" 2, 041712
dc.description.abstractX-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.
dc.description.sponsorshipR.S. thanks the Graduate School for Computing in Medicine and Life Sciences at the University of Lübeck for funding. L.R., M.K., D.R., and C.B. thank the German Federal Ministry for Education and Research (BMBF) for funding (Grant Nos. 01KX0806 and 01KX0807). L.R., M.D., and C.B. acknowledge support from the BMBF in the context of the Röntgen-Angström-Cluster (Grant No. 05K12GU3). J.M.-C. and A.B.-N. acknowledge support from the Spanish Ministerio Economía y Competitividad (MINECO, Grant No. BFU2013-43513-R). I.V.M., R.D., and L.R. are grateful for support from the DFG Cluster of Excellence “Inflammation at Interfaces” (EXC 306)
dc.publisherAIP Publishing
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-43513-R/ES/REOVIRUS AVIAR: FACTORES DE VIRULENCIA Y NUEVAS DIANAS TERAPEUTICAS
dc.rightsCopyright © 2015, Author(s). This article is distributed under a Creative Commons Attribution 3.0 Unported license
dc.titleReal-time investigation of dynamic protein crystallization in living cells
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular

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Copyright © 2015, Author(s). This article is distributed under a  Creative Commons Attribution 3.0 Unported license
Except where otherwise noted, this item's license is described as  Copyright © 2015, Author(s). This article is distributed under a Creative Commons Attribution 3.0 Unported license

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