Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
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Título: | Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides |
Autor/a: | Mosquera Mosquera, Jesús Jiménez Balsa, Adrián Dodero, Verónica Isabel Vázquez Sentís, Marco Eugenio Mascareñas Cid, José Luis |
Centro/Departamento: | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares Universidade de Santiago de Compostela. Departamento de Química Orgánica |
Palabras chave: | DNA | Synthetic bZIP peptides | |
Data: | 2013 |
Editor: | Nature Publishing Group |
Cita bibliográfica: | Mosquera Mosquera, J., Jimenez Balsa, A., Dodero, V.I., Vázquez Sentís, M.E. y Mascareñas Cid, J.L.(2013). Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides. Nat. commun., vol.4:1874 |
Resumo: | One of the strategies used by nature to regulate gene expression relies on the stimulicontrolled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be directed towards two different DNA sequences depending on its dimerization arrangement. While the monomeric peptide is non-functional, a C-terminal metallo-dimer recognizes the natural ATF/CREB-binding site (50 -ATGA cg TCAT-30 ), and a N-terminal disulphide dimer binds preferentially to the swapped sequence (50 -TCAT cg ATGA-30 ). As the dimerization mode can be efficiently controlled by appropriate external reagents, it is possible to reversibly drive the peptide to either DNA site in response to such specific inputs. This represents the first example of a designed molecule that can bind to more than one specific DNA sequence depending on changes in its environment |
Versión do editor: | https://doi.org/10.1038/ncomms2825 |
URI: | http://hdl.handle.net/10347/22210 |
DOI: | 10.1038/ncomms2825 |
ISSN: | 2041-1723 |
Dereitos: | © 2013 Macmillan Publishers Limited |
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