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dc.contributor.authorRegal López, Patricia
dc.contributor.authorDíaz Bao, Mónica
dc.contributor.authorBarreiro Tomé, Rocío
dc.contributor.authorFente Sampayo, Cristina Asunción
dc.contributor.authorCepeda Sáez, Alberto
dc.date.accessioned2020-05-20T17:18:39Z
dc.date.available2020-05-20T17:18:39Z
dc.date.issued2017
dc.identifier.citationRegal, P.; Díaz-Bao, M.; Barreiro, R.; Fente, C.; Cepeda, A. Design of a Molecularly Imprinted Stir-Bar for Isolation of Patulin in Apple and LC-MS/MS Detection. Separations 2017, 4, 11.
dc.identifier.urihttp://hdl.handle.net/10347/22495
dc.description.abstractMycotoxins are a very diverse group of natural products produced as secondary metabolites by fungi. Patulin is produced by mold species normally related to vegetable-based products and fruit, mainly apple. Its ingestion may result in agitation, convulsions, edema, intestinal ulceration, inflammation, vomiting, and even immune, neurological or gastrointestinal disorders. For this reason, the European Commission Regulation (EC) 1881/2006 established a maximum content for patulin of 10 ppb in infant fruit juice, 50 ppb for fruit juice for adults and 25 ppb in fruit-derived products. In this work, a rapid and selective method based on magnetic molecularly imprinted stir-bar (MMISB) extraction has been developed for the isolation of patulin, using 2-oxindole as a dummy template. The final extraction protocol consisted of simply pouring in, stirring and pouring out samples and solvents from a beaker with the MMISB acting inside. The magnetic device provided satisfactory recoveries of patulin (60%–70%) in apple samples. The successful MMISB approach has been combined with high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to determine patulin.
dc.description.sponsorshipThis research was supported by the project EM 2012/153 from Consellería de Cultura, Educación e Ordenacion Universitaria, Xunta de Galicia
dc.language.isoeng
dc.publisherMDPI
dc.rights© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPatulin
dc.subjectMolecularly imprinted polymers (MIP)
dc.subjectStir-bar
dc.subjectApple
dc.subjectHPLC-MS/MS
dc.titleDesign of a molecularly imprinted stir-bar for isolation of patulin in apple and LC-MS/MS detection
dc.typejournal article
dc.identifier.doi10.3390/separations4020011
dc.relation.publisherversionhttps://doi.org/10.3390/separations4020011
dc.type.hasVersionVoR
dc.identifier.essn2297-8739
dc.rights.accessRightsopen access
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía
dc.description.peerreviewedSI


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© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
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 © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)





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