Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic Escherichia coli strains with different uncommon intimin types
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Título: | Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic Escherichia coli strains with different uncommon intimin types |
Autor/a: | Yamamoto, Denise Hernandes, Rodrigo T. Blanco Álvarez, Miguel Greune, Lilo Schmidt, M. Alexander Carneiro, Sylvia M. Dahbi, Ghizlane Blanco Álvarez, Jesús Eulogio Mora Gutiérrez, Azucena Blanco Álvarez, Jorge Tardelli Gomes, Tânia Aparecida |
Centro/Departamento: | Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía |
Palabras chave: | Tight Junction | Invasion Index | Tight Junction Disruption | aEPEC Strain | Additional Virulence Gene | |
Data: | 2009 |
Editor: | BMC |
Cita bibliográfica: | Yamamoto, D., Hernandes, R.T., Blanco, M. et al. Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic Escherichia colistrains with different uncommon intimin types. BMC Microbiol 9, 146 (2009). https://doi.org/10.1186/1471-2180-9-146 |
Resumo: | Background Enteropathogenic Escherichia coli (EPEC) produce attaching/effacing (A/E) lesions on eukaryotic cells mediated by the outer membrane adhesin intimin. EPEC are sub-grouped into typical (tEPEC) and atypical (aEPEC). We have recently demonstrated that aEPEC strain 1551-2 (serotype O non-typable, non-motile) invades HeLa cells by a process dependent on the expression of intimin sub-type omicron. In this study, we evaluated whether aEPEC strains expressing other intimin sub-types are also invasive using the quantitative gentamicin protection assay. We also evaluated whether aEPEC invade differentiated intestinal T84 cells. Results Five of six strains invaded HeLa and T84 cells in a range of 13.3%–20.9% and 5.8%–17.8%, respectively, of the total cell-associated bacteria. The strains studied were significantly more invasive than prototype tEPEC strain E2348/69 (1.4% and 0.5% in HeLa and T84 cells, respectively). Invasiveness was confirmed by transmission electron microscopy. We also showed that invasion of HeLa cells by aEPEC 1551-2 depended on actin filaments, but not on microtubules. In addition, disruption of tight junctions enhanced its invasion efficiency in T84 cells, suggesting preferential invasion via a non-differentiated surface. Conclusion Some aEPEC strains may invade intestinal cells in vitro with varying efficiencies and independently of the intimin sub-type. |
Versión do editor: | https://doi.org/10.1186/1471-2180-9-146 |
URI: | http://hdl.handle.net/10347/22742 |
DOI: | 10.1186/1471-2180-9-146 |
ISSN: | 1471-2180 |
Dereitos: | © 2009 Yamamoto et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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© 2009 Yamamoto et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
© 2009 Yamamoto et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.