Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilage
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Título: | Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilage |
Autor/a: | Pombo Suárez, Manuel Enrique Calaza Cabanas, Manuel Gómez-Reino Carnota, Juan Jesús González Martínez-Pedrayo, Antonio |
Centro/Departamento: | Universidade de Santiago de Compostela. Departamento de Psiquiatría, Radioloxía, Saúde Pública, Enfermaría e Medicina |
Palabras chave: | Reference Gene | Articular Cartilage | Subchondral Bone | Stable Gene | Candidate Reference Gene | |
Data: | 2008 |
Editor: | BMC |
Cita bibliográfica: | Pombo-Suarez, M., Calaza, M., Gomez-Reino, J.J. et al. Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilage. BMC Molecular Biol 9, 17 (2008). https://doi.org/10.1186/1471-2199-9-17 |
Resumo: | Background Assessment of gene expression is an important component of osteoarthritis (OA) research, greatly improved by the development of quantitative real-time PCR (qPCR). This technique requires normalization for precise results, yet no suitable reference genes have been identified in human articular cartilage. We have examined ten well-known reference genes to determine the most adequate for this application. Results Analyses of expression stability in cartilage from 10 patients with hip OA, 8 patients with knee OA and 10 controls without OA were done with classical statistical tests and the software programs geNorm and NormFinder. Results from the three methods of analysis were broadly concordant. Some of the commonly used reference genes, GAPDH, ACTB and 18S RNA, performed poorly in our analysis. In contrast, the rarely used TBP, RPL13A and B2M genes were the best. It was necessary to use together several of these three genes to obtain the best results. The specific combination depended, to some extent, on the type of samples being compared. Conclusion Our results provide a satisfactory set of previously unused reference genes for qPCR in hip and knee OA This confirms the need to evaluate the suitability of reference genes in every tissue and experimental situation before starting the quantitative assessment of gene expression by qPCR |
Versión do editor: | https://doi.org/10.1186/1471-2199-9-17 |
URI: | http://hdl.handle.net/10347/22944 |
DOI: | 10.1186/1471-2199-9-17 |
ISSN: | 1471-2199 |
Dereitos: | © 2008 Pombo-Suarez et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
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© 2008 Pombo-Suarez et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
© 2008 Pombo-Suarez et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited