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dc.contributor.authorSeoane Ruzo, Samuel
dc.contributor.authorÁlvarez Bermúdez, María José
dc.contributor.authorSendón Lago, Juan José
dc.contributor.authorMartínez Ordóñez, Anxo
dc.contributor.authorAbdul-Hadi, Soraya
dc.contributor.authorMaestro Saavedra, Miguel Anxo
dc.contributor.authorMouriño Mosquera, Antonio
dc.contributor.authorPérez Fernández, Román
dc.date.accessioned2020-06-16T07:56:02Z
dc.date.available2020-06-16T07:56:02Z
dc.date.issued2013
dc.identifier.citationSeoane, S.; Bermudez, M.A.; Sendon-Lago, J.; Martinez-Ordoñez, A.; Abdul-Hadi, S.; Maestro, M.; Mouriño, A.; Perez-Fernandez, R. 26,26,26,27,27,27-Hexadeuterated-1,25-Dihydroxyvitamin D3 (1,25D-d6) As Adjuvant of Chemotherapy in Breast Cancer Cell Lines. Cancers 2014, 6, 67-78
dc.identifier.urihttp://hdl.handle.net/10347/23007
dc.description.abstractIt has been demonstrated that 1,25-dihydroxyvitamin D3 (1,25D) and some of its analogues have antitumor activity. 1,25D labeled with deuterium (26,26,26,27,27,27-hexadeuterated 1a,25-dihydroxyvitamin D3, or 1,25D-d6) is commonly used as internal standard for 1,25D liquid chromatography-mass spectrometry (LC-MS) quantification. In the present study using human breast cancer cell lines, the biological activity of 1,25D-d6 administered alone and in combination with two commonly used antineoplastic agents, 5-fluorouracil and etoposide, was evaluated. Using an MTT assay, flow cytometry, and western blots, our data demonstrated that 1,25D-d6 has effects similar to the natural hormone on cell proliferation, cell cycle, and apoptosis. Furthermore, the combination of 1,25D-d6 and etoposide enhances the antitumoral effects of both compounds. Interestingly, the antitumoral effect is higher in the more aggressive MDA-MB-231 breast cancer cell line. Our data indicate that 1,25D-d6 administered alone or in combination with chemotherapy could be a good experimental method for accurately quantifying active 1,25D levels in cultures or in biological fluids, on both in vitro breast cancer cell lines and in vivo animal experimental models
dc.description.sponsorshipSamuel Seoane is a fellow from Fundación Científica de la Asociación Española Contra el Cáncer. This study was supported by a grant to R.P.-F. from Ministerio de Economía y Competividad (SAF2012-38240), and to A.M. from Ministerio de Educación e Innovación (MEI) (SAF2010–15291), and Xunta de Galicia (CN2012/074, INCITE08PXIB209130PR)
dc.language.isoeng
dc.publisherMDPI
dc.rights© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subject1a,25-dihydroxyvitamin D3
dc.subject26,26,26,27,27,27-hexadeuterated 1a
dc.subject25-dihydroxyvitamin D3
dc.subjectVitamin D
dc.subjectDeuterated vitamin D
dc.subjectChemotheraphy
dc.subjectBreast cancer
dc.title26,26,26,27,27,27-Hexadeuterated-1,25-Dihydroxyvitamin D3 (1,25D-d6) As Adjuvant of Chemotherapy in Breast Cancer Cell Lines
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.3390/cancers6010067
dc.relation.publisherversionhttps://doi.org/10.3390/cancers6010067
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn2072-6694
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Fisioloxía
dc.description.peerreviewedSI


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© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)
This item's license is described as
 © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)





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