Production of microbial transglutaminase on media made from sugar cane molasses and glycerol
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Título: | Production of microbial transglutaminase on media made from sugar cane molasses and glycerol |
Autor/a: | Portilla Rivera, Óscar M. Téllez Luis, Simón J. Ramírez de León, José Alberto Vázquez Vázquez, Manuel |
Centro/Departamento: | Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía |
Palabras chave: | Transglutaminase | Glycerol | Glucose | Sugar cane molasses | |
Data: | 2009 |
Editor: | University of Zagreb |
Cita bibliográfica: | Portilla Rivera, Oscar M. ; Téllez Luis, Simón J. ; Ramírez de León, José A. ; Vázquez, Manuel. (2009). Production of Microbial Transglutaminase. "Food Technology Biotechnology", vol. 47, n. 1, p. 19–26 |
Resumo: | Transglutaminase is an enzyme that catalyses an acyl transfer reaction between γ-carboxamide groups of glutaminyl residues and lysine residues in proteins. Due to this property, this enzyme is used for enhancing textural properties of protein-rich food. The transglutaminase used as food additive is obtained by microorganisms, mainly by Streptoverticillium ladakanum. On the other hand, sugar cane molasses is a viscous liquid rich in noncrystallized carbohydrates (saccharose, glucose and fructose). In this work, the feasibility of using sugar cane molasses as a carbon source for the production of microbial transglutaminase by Streptoverticillium ladakanum NRRL 3191 has been studied. Carbon sources including sugar cane molasses (60 g of total sugars per L), glycerol (60 g/L) and their mixture in a ratio of 1:1 (30 g/L of each) were evaluated. Time course of microbial growth, transglutaminase activity and carbon source consumption were determined every 24 h during 120 h of fermentations at three agitation speeds (200, 300 or 400 rpm). The results showed that with the increase in agitation speed, the biomass concentration increased up to 8.39 g/L in the medium containing sugar cane molasses alone or the mixture of molasses and glycerol. The highest transglutaminase activity was obtained at 400 rpm in the medium containing a mixture of molasses and glycerol, reaching 0.460 U/mL, while in the medium containing sugar cane molasses alone, the activity was 0.240 U/mL, and using glycerol alone it was 0.250 U/mL. These results show that sugar cane molasses is a suitable medium for transglutaminase production when it is combined with glycerol |
Versión do editor: | https://www.ftb.com.hr/archives/66-volume-47-issue-no-1/230-production-of-microbial-transglutaminase-on-media-made-from-sugar-cane-molasses-and-glycerol |
URI: | http://hdl.handle.net/10347/23048 |
ISSN: | 1330-9862 |
E-ISSN: | 1334-2606 |
Dereitos: | © 2007 by the authors. Licensee Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) |
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© 2007 by the authors. Licensee Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
© 2007 by the authors. Licensee Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)