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dc.contributor.authorMartínez Matamoros, Diana
dc.contributor.authorCastro García, Socorro
dc.contributor.authorBalado Dacosta, Miguel
dc.contributor.authorMatamoros Veloza, Adriana
dc.contributor.authorCamargo Valero, Miller Alonso
dc.contributor.authorCespedes, Oscar
dc.contributor.authorRodríguez, Jaime
dc.contributor.authorJiménez, Carlos
dc.contributor.authorLemos Ramos, Manuel Luis
dc.date.accessioned2020-07-13T08:55:04Z
dc.date.available2020-07-13T08:55:04Z
dc.date.issued2019
dc.identifier.citationMartínez Matamoros, D., Castro García, S., Balado Dacosta, M., Matamoros Veloza, A., Camargo Valero, M.A., Cespedes, O. et al. (2019). Preparation of functionalized magnetic nanoparticles conjugated with feroxamine and their evaluation for pathogen detection. RSC Advances, vol. 9, 13533-13542
dc.identifier.issn2046-2069
dc.identifier.urihttp://hdl.handle.net/10347/23119
dc.description.abstractThis work reports the preparation of a conjugate between amino-functionalized silica magnetite and thesiderophore feroxamine. The morphology and properties of the conjugate and intermediate magneticnanoparticles (MNPs) were examined by powder X-ray diffraction (XRD), Fourier Transform Infraredspectroscopy (FT-IR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), magnetizationstudies, zeta potential measurements, Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX) mapping. Furthermore, this study investigated the interaction between the functionalizedmagnetic NPs andYersinia enterocoliticawild type (WC-A) using Scanning Electron Microscopy (SEM)and TEM images. In addition, the interaction between MNPs and aY. enterocoliticamutant strain lackingferoxamine receptor FoxA, was also used to study the binding specificity. The results showed that thecapture and isolation ofY. enterocoliticaby the MNPs took place in all cases. Moreover, the specificinteraction between the MNP conjugate and bacteria did not increase after blocking the free aminegroups witht-butoxycarbonyl (Boc) and carboxylic acid (COOH) functional groups. Electrostatic surfaceinteractions instead of molecular recognition between MNP conjugate and feroxamine receptor seem torule the attachment of bacteria to the conjugate
dc.description.sponsorshipThis work was supported by grants AGL2015-63740-C2-2-R and AGL2015-63740-C2-1-R (AEI/FEDER, EU) from the State Agency for Research (AEI) of Spain, both co-funded by the FEDER Programme from the European Union
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.rights© The Royal Society of Chemistry 2019. Open Access Article. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
dc.subjectNanoparticles
dc.subjectFeroxamine
dc.subjectPathogen
dc.titlePreparation of functionalized magnetic nanoparticles conjugated with feroxamine and their evaluation for pathogen detection
dc.typejournal article
dc.identifier.doi10.1039/c8ra10440a
dc.relation.publisherversionhttps://doi.org/10.1039/C8RA10440A
dc.type.hasVersionVoR
dc.rights.accessRightsopen access
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Acuicultura
dc.description.peerreviewedSI


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© The Royal Society of Chemistry 2019. Open Access Article. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence
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 © The Royal Society of Chemistry 2019. Open Access Article. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence





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