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dc.contributor.authorMamani Huarani, Rosalía Seferina
dc.contributor.authorFlament Simon, Saskia Camille
dc.contributor.authorGarcía Menéndez, Vanesa
dc.contributor.authorMora Gutiérrez, Azucena
dc.contributor.authorAlonso, María Pilar
dc.contributor.authorLópez Capón, Cecilia
dc.contributor.authorGarcía Meniño, Isidro
dc.contributor.authorDíaz Jiménez, Dafne
dc.contributor.authorBlanco Álvarez, Jesús Eulogio
dc.contributor.authorBlanco Álvarez, Miguel
dc.contributor.authorBlanco Álvarez, Jorge
dc.date.accessioned2020-07-17T07:30:00Z
dc.date.available2020-07-17T07:30:00Z
dc.date.issued2019
dc.identifier.citationMamani R, Flament-Simon SC, García V, Mora A, Alonso MP, López C, García-Meniño I, Díaz-Jiménez D, Blanco JE, Blanco M and Blanco J (2019) Sequence Types, Clonotypes, Serotypes, and Virotypes of Extended-Spectrum β-Lactamase-Producing Escherichia coli Causing Bacteraemia in a Spanish Hospital Over a 12-Year Period (2000 to 2011). Front. Microbiol. 10:1530. doi: 10.3389/fmicb.2019.01530
dc.identifier.urihttp://hdl.handle.net/10347/23172
dc.description.abstractThe aim of the present study was to examine the prevalence and determine the molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) causing bacteraemia in a Spanish Hospital over a 12-year period (2000 to 2011). As far as we know, this is the first study which has investigated and compared the serotypes, phylogroups, clonotypes, virotypes, and PFGE profiles of ST131 and non-ST131 clones of bacteraemia ESBL-EC isolates. Of the 2,427 E. coli bloodstream isolates, 96 (4.0%) were positive for ESBL production: 40 for CTX-M-15, 36 for CTX-M-14, eight for CTX-M-1, four for CTX-M-9, CTX-M-32, and SHV-12. The number of ESBL-EC increased from 1.0% during 2000 to 2005 to 5.5% during 2006–2011 (P < 0.001). The 96 ESBL-EC isolates belonged to 36 different STs. The commonest was ST131 (41 isolates), followed by ST58, ST354, ST393 and ST405 (four isolates each). Most CTX-M-15 isolates (87.5%, 35/40) were ST131, whereas the 36 CTX-M-14 isolates belonged to 23 different STs and only 3 (8.3%) of them were ST131. The 35 ST131 CTX-M-15-producing isolates belonged to the H30Rx subclone and 29 of them showed the virotype A. A drastic change in ST131 virotypes happened in 2011 due to the emergence of the virotypes E (sat, papGII, cnf1, hlyA, and kpsMII-K5) and F (sat, papGII, and kpsMII-K5) which displaced virotype A (afa/draBC, afa operon FM955459, sat, and kpsMII-K2). Although the 96 ESBL-EC isolates showed 21 O serogroups and 17 H flagellar antigens, 39 belonged to serotype O25b:H4 (ST131 isolates). The second most prevalent serotype (O15:H1) was found to be associated with another important high-risk clone (ST393). In conclusion, the ST131 was the most frequent sequence type, being the H30Rx subclone responsible for the significant increase of ESBL-EC isolates since 2006. Here, we report two new virotypes (E and F) of the H30Rx subclone emerged in 2011. Future molecular studies are needed to understand the dynamics of expansion of this successful high-risk subclone in order to prevent its spread and establish the importance of the two new virotypes
dc.description.sponsorshipThis study was supported by the following projects: PI16/01477 from the Plan Estatal de I+D+I 2013-2016, Instituto de Salud Carlos III (ISCIII), Subdirección General de Evaluación y Fomento de la Investigación, and Fondo Europeo de Desarrollo Regional (FEDER); AGL2013-47852-R from the Spanish Ministerio de Economía y Competitividad (MINECO) and FEDER; AGL2016-79343-R from the Spanish Agencia Estatal de Investigación (AEI) and FEDER; and CN2012/303 and ED431C2017/57 from the Consellería de Cultura, Educación e Ordenación Universitaria, (Xunta de Galicia) and FEDER
dc.language.isoeng
dc.publisherFrontiers Media
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-47852-R/ES/FUENTES DE TRANSMISION DEL GRUPO CLONAL PANDEMICO ST131 DE ESCHERICHIA COLI. CARACTERIZACION MOLECULAR DEL PATOGENO Y DE SU VIRULENCIA IN VIVO, EVALUACION DE RIESGO Y CONTROL
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-79343-R/ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI16-01477/ES/Caracterización molecular de clones de Escherichia coli de alto riesgo (ST131 y otros) causantes de infecciones extraintestinales en seres humanos. Los animales como reservorio
dc.rightsCopyright © 2019 Mamani, Flament-Simon, García, Mora, Alonso, López, García-Meniño, Díaz-Jiménez, Blanco, Blanco and Blanco. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
dc.subjectClonotypes
dc.subjectE. coli
dc.subjectESBL
dc.subjectExPEC
dc.subjectH30Rx subclone
dc.subjectST131 clonal group
dc.titleSequence types, clonotypes, serotypes, and virotypes of extended-spectrum beta-lactamase-producing Escherichia coli causing bacteraemia in a Spanish hospital over a 12-year period (2000 to 2011)
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.3389/fmicb.2019.01530
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2019.01530
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn1664-302X
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.description.peerreviewedSI


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