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dc.contributor.authorMartínez Negro, María
dc.contributor.authorGuerrero Martínez, Andrés
dc.contributor.authorGarcía Río, Luis
dc.contributor.authorDomènech, Òscar
dc.contributor.authorAicart, Emilio
dc.contributor.authorTros de Ilarduya, Conchita
dc.contributor.authorJunquera, Elena
dc.identifier.citationACS Omega 2018, 3, 1, 208–217
dc.description.abstractA multidisciplinary strategy, including bothbiochemical and biophysical studies, was proposed here toevaluate the potential of lipid nanoaggregates consisting of amixture of a gemini−bolaamphiphilic lipid (C6C22C6) and thewell-known helper lipid 1,2-dioleoyl-sn-glycero-3-phosphatidy-lethanolamine (DOPE) to transfect plasmid DNA into livingcells in an efficient and safe way. For that purpose, severalexperimental techniques were employed, such as zeta potential(phase analysis light scattering methodology), agarose gelelectrophoresis (pDNA compaction and pDNA protectionassays), small-angle X-ray scattering, cryo-transmission electronmicroscopy, atomic force microscopy,fluorescence-assisted cellsorting, luminometry, and cytotoxicity assays. The resultsrevealed that the cationic lipid and plasmid offer only 70 and30% of their nominal positive (=++q2.0nom,C C C6226) and negative charges (=−−q2/bpnom,pDNA), respectively. Upon mixing withDOPE, they form lipoplexes that self-aggregate in typical multilamellar Lαlyotropic liquid-crystal nanostructures with sizes in therange of 100−200 nm and low polydispersities, very suitablyfitted to remain in the bloodstream and cross the cell membrane.Interestingly, these nanoaggregates were able to compact, protect (from the degrading effect of DNase I), and transfect two DNAplasmids (pEGFP-C3, encoding the greenfluorescent protein, and pCMV-Luc, encoding luciferase) into COS-7 cells, with anefficiency equal or even superior to that of the universal control Lipo2000*, as long as the effective +/−charge ratio wasmaintained higher than 1 but reasonably close to electroneutrality. Moreover, this transfection process was not cytotoxic becausethe viability of COS-7 cells remained at high levels, greater than 80%. All of these features make the C6C22C6/DOPE nanosysteman optimal nonviral gene nanocarrier in vitro and a potentially interesting candidate for future in vivo experiments
dc.description.sponsorshipFinancial support from the Ministerio de Economia y Competitividad of Spain (projects CTQ2012-30821, CTQ2015-65972-R, CTQ2015-64425-C2-2-R, and CTQ2014-55208-P), Madrid Regional Government (S2013/MIT-2807), Xunta de Galicia (GR 2007/085; IN607C 2016/03 and Centro Singular de Investigación de Galicia accreditation 2016–2019, ED431G/09), the European Regional Development Fund (ERDF), and Universidad Complutense de Madrid, Spain (project UCMA05-33-010), is gratefully acknowledged
dc.publisherAmerican Chemical Society
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-55208-P/ES/ENSAMBLAJE DE SISTEMAS SUPRAMOLECULARES Y SU INFLUENCIA SOBRE LA REACTIVIDAD QUIMICA
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-65972-R/ES/MACROCICLOS POLICATIONICOS COMO NANOVECTORES DE ACIDOS NUCLEICOS (PDNAS Y SIRNAS): UN PLANTEAMIENTO PLURIDISCIPLINAR EN TERAPIA GENICA
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-64425-C2-2-R/ES/DISEÑO, DESARROLLO Y EVALUACION DE NUEVOS SISTEMAS DENDRIMERICOS Y GLICODENDRIMERICOS DE APLICACION A TERAPIA GENICA ANTITUMORAL
dc.rightsCopyright © 2018 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permitscopying and redistribution of the article or any adaptations for non-commercial purposes (
dc.subjectChemical structure
dc.titleMultidisciplinary approach to the transfection of plasmid DNA by a nonviral nanocarrier based on a Gemini-Bolaamphiphilic hybrid lipid
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Física

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