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dc.contributor.authorMartínez Ledo, Adriana
dc.contributor.authorSenra, Ana
dc.contributor.authorRilo Álvarez, Héctor
dc.contributor.authorBorrajo Alonso, Erea
dc.contributor.authorVidal Figueroa, Anxo
dc.contributor.authorAlonso Fernández, María José
dc.contributor.authorGarcía Fuentes, Marcos
dc.date.accessioned2020-07-31T10:50:11Z
dc.date.issued2020
dc.identifier.citationLedo, A. M., Senra, A., Rilo-Alvarez, H., Borrajo, E., Vidal, A., Alonso, M. J., & Garcia-Fuentes, M. (2020). mRNA-activated matrices encoding transcription factors as primers of cell differentiation in tissue engineering. Biomaterials, 247, 120016-120016.
dc.identifier.issn0142-9612
dc.identifier.urihttp://hdl.handle.net/10347/23240
dc.description.abstractGene-activated matrices (GAMs) encoding pivotal transcription factors (TFs) represent a powerful tool to direct stem cell specification for tissue engineering applications. However, current TF-based GAMs activated with pDNA, are challenged by their low transfection efficiency and delayed transgene expression. Here, we report a GAM technology activated with mRNAs encoding TFs SOX9 (cartilage) and MYOD (muscle). We find that these mRNA-GAMs induce a higher and faster TF expression compared to pDNA-GAMs, especially in the case of RNase resistant mRNA sequences. This potent TF expression was translated into a high synthesis of cartilage- and muscle-specific markers, and ultimately, into successful tissue specification in vitro. Additionally, we show that the expression of tissue-specific markers can be further modulated by altering the properties of the mRNA-GAM environment. These results highlight the value of this GAM technology for priming cell lineage specification, a key centerpiece for future tissue engineering devices.
dc.description.sponsorshipThis work has been funded by Ministerio de Economía y Competitividad (MINECO-RETOS, Grant MAT2017-84361-R, Feder Funds), Fundación BBVA 2014-PO0110 and Xunta de Galicia (Grupos de Referencia Competitiva, Feder Funds; Convenio para fomentar a actividade investigadora do persoal investigador finalista nas convocatorias de axudas do ERC no marco da H2020). AML was a recipient of a FPU grant from Ministerio de Economía y Competitividad (FPU12/05528)
dc.language.isoeng
dc.publisherElsevier
dc.rights© 2020 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectGAMs
dc.subject3D transfection
dc.subjectmRNA
dc.subjectTranscription factors
dc.subjectLineage priming
dc.subjectTissue engineering
dc.titlemRNA-activated matrices encoding transcription factors as primers of cell differentiation in tissue engineering
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1016/j.biomaterials.2020.120016
dc.relation.publisherversionhttps://doi.org/10.1016/j.biomaterials.2020.120016
dc.description.embargo2022-03-31
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/embargoedAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Fisioloxía
dc.description.peerreviewedSI


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© 2020 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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 © 2020 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)





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