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dc.contributor.authorCoco, Laura del
dc.contributor.authorMajellaro, María
dc.contributor.authorBoccarelli, Angelina
dc.contributor.authorCellamare, Saverio
dc.contributor.authorAltomare, Cosimo Damiano
dc.contributor.authorFanizzi, Francesco Paolo
dc.date.accessioned2020-11-02T12:55:54Z
dc.date.available2020-11-02T12:55:54Z
dc.date.issued2020
dc.identifier.citationDel Coco, L.; Majellaro, M.; Boccarelli, A.; Cellamare, S.; Altomare, C.D.; Fanizzi, F.P. Novel Antiproliferative Biphenyl Nicotinamide: NMR Metabolomic Study of its Effect on the MCF-7 Cell in Comparison with Cisplatin and Vinblastine. Molecules 2020, 25, 3502
dc.identifier.urihttp://hdl.handle.net/10347/23528
dc.description.abstractA 1H-NMR-based metabolomic study was performed on MCF-7 cell lines treated with a novel nicotinamide derivative (DT-8) in comparison with two drugs characterized by a well-established mechanism of action, namely the DNA-metalating drug cisplatin (cis-diamminedichloridoplatinum(II), CDDP) and the antimitotic drug vinblastine (vinblastine, VIN). The effects of the three compounds, each one at the concentration corresponding to the IC50 value, were investigated, with respect to the controls (K), by the 1H-NMR of cells lysates and multivariate analysis (MVA) of the spectroscopic data. Relevant differences were found in the metabolic profiles of the different treatments with respect to the controls. A large overlap of the metabolic profiles in DT-8 vs. K and VIN vs. K suggests a similar biological response and mechanism of action, significantly diverse with respect to CDDP. On the other hand, DT8 seems to act by disorganizing the mitotic spindle and ultimately blocking the cell division, through a mechanism implying methionine depletion and/or S-adenosylmethionine (SAM) limitation
dc.description.sponsorshipThis research was funded by PRIN, Grant 201744BN5T_004
dc.language.isoeng
dc.publisherMDPI
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBiaryl nicotinamide
dc.subjectBreast cancer
dc.subjectAntiproliferative activity
dc.subjectMetabolomics
dc.subjectNMR spectroscopy
dc.subjectMVA
dc.titleNovel Antiproliferative Biphenyl Nicotinamide: NMR Metabolomic Study of its Effect on the MCF-7 Cell in Comparison with Cisplatin and Vinblastine
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.3390/molecules25153502
dc.relation.publisherversionhttps://doi.org/10.3390/molecules25153502
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn1420-3049
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.description.peerreviewedSI


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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Except where otherwise noted, this item's license is described as  © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)





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