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dc.contributor.authorCrespo Vieira, Andrés
dc.contributor.authorRubiolo Gaytán, Juan Andrés
dc.contributor.authorLópez Alonso, Henar
dc.contributor.authorCifuentes Martínez, José Manuel
dc.contributor.authorAlfonso Rancaño, María Amparo
dc.contributor.authorBermúdez Pose, Roberto
dc.contributor.authorOtero Fuertes, María Paz
dc.contributor.authorRodríguez Vieytes, María Mercedes
dc.contributor.authorVega Lisi, Félix Víctor
dc.contributor.authorBotana López, Luis Miguel
dc.date.accessioned2020-11-06T12:56:26Z
dc.date.available2020-11-06T12:56:26Z
dc.date.issued2013
dc.identifier.citationVieira, A.C.; Rubiolo, J.A.; López-Alonso, H.; Cifuentes, J.M.; Alfonso, A.; Bermúdez, R.; Otero, P.; Vieytes, M.R.; Vega, F.V.; Botana, L.M. Oral Toxicity of Okadaic Acid in Mice: Study of Lethality, Organ Damage, Distribution and Effects on Detoxifying Gene Expression. Toxins 2013, 5, 2093-2108
dc.identifier.urihttp://hdl.handle.net/10347/23597
dc.description.abstractIn vivo, after administration by gavage to mice and rats, okadaic acid has been reported to produce lesions in liver, small intestine and forestomach. Because several reports differ in the damage detected in different organs, and on okadaic acid distribution after consumption, we determined the toxicity of this compound after oral administration to mice. After 24 hours, histopathological examination showed necrotic foci and lipid vacuoles in the livers of intoxicated animals. By immunohistochemical analysis, we detected this toxin in the liver and kidneys of intoxicated animals. Okadaic acid induces oxidative stress and can be activated in vitro into reactive compounds by the post-mitochondrial S9 fraction, so we studied the okadaic effect on the gene expression of antioxidant and phase II detoxifying enzymes in liver. We observed a downregulation in the expression of these enzymes and a reduction of protein expression of catalase and superoxide dismutase 1 in intoxicated animals
dc.description.sponsorshipThe research leading to these results has received funding from the following FEDER cofunded-grants: From Ministerio de Ciencia y Tecnología, Spain: AGL2009-13581-CO2-01, AGL2012-40485-CO2-01. From Xunta de Galicia, Spain: 10PXIB261254 PR. From the European Union’s Seventh Framework Programme managed by REA–Research Executive Agency (FP7/2007-2013) under grant agreement Nos. 265896 BAMMBO, 265409 µAQUA, and 262649 BEADS, 315285 Ciguatools and 312184 PharmaSea. From the Atlantic Area Programme (Interreg IVB Trans-national): 2009-1/117 Pharmatlantic
dc.language.isoeng
dc.publisherMDPI
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/265896
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/265409
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/262649
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/315285
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/312184
dc.relationinfo:eu-repo/grantAgreement/MICINN/Plan Nacional de I+D+i 2008-2011/AGL2009-13581-C02-01/ES/Evaluacion De Los Riesgos De Salud Publica Debidos A Toxinas Marinas De Aguas Templadas En Las Costas Europeas Y Del Efecto Sinergico Con Otras Toxinas Habituales En Estas Latitudes. Subproyecto 2
dc.rights© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectOkadaic acid
dc.subjectHistopathology
dc.subjectDiarrhea
dc.subjectImmunohistochemistry
dc.subjectLiver
dc.subjectSuperoxide dismutase 1
dc.subjectCatalase
dc.subjectQuantitative PCR
dc.subjectMice
dc.titleOral Toxicity of Okadaic Acid in Mice: Study of Lethality, Organ Damage, Distribution and Effects on Detoxifying Gene Expression
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.3390/toxins5112093
dc.relation.publisherversionhttps://doi.org/10.3390/toxins5112093
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn2072-6651
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Fisioloxía
dc.description.peerreviewedSI


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© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)
Except where otherwise noted, this item's license is described as  © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)





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