Clonal in vitro propagation of peat mosses (Sphagnum L.) as novel green resources for basic and applied research
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xmlui.metadata.dc.title: | Clonal in vitro propagation of peat mosses (Sphagnum L.) as novel green resources for basic and applied research |
xmlui.metadata.dc.contributor.author: | Beike, Anna K. Spagnuolo, Valeria Lüth, Volker Steinhart, Feray Ramos Gómez, Julia Krebs, Matthias Adamo, Paola Rey Asensio, Ana Isabel Fernández Escribano, José Ángel Giordano, Simonetta Decker, Eva L. Reski, Ralf |
xmlui.metadata.dc.contributor.affiliation: | Universidade de Santiago de Compostela. Departamento de Bioloxía Funcional |
xmlui.metadata.dc.subject: | Biomonitoring | In vitro cultivation | Moss bag technique | Mosses | Sphagnum | Sphagnum propagule | |
xmlui.metadata.dc.date.issued: | 2015 |
xmlui.metadata.dc.publisher: | Springer |
xmlui.metadata.dc.identifier.citation: | Beike, A.K., Spagnuolo, V., Lüth, V. et al. Clonal in vitro propagation of peat mosses (Sphagnum L.) as novel green resources for basic and applied research. Plant Cell Tiss Organ Cult 120, 1037–1049 (2015). https://doi.org/10.1007/s11240-014-0658-2 |
xmlui.metadata.dc.description.abstract: | As builders and major components of peatlands, Sphagnopsida (peat mosses) are very important organisms for ecosystems and world’s climate. Nowadays many Sphagnum species as well as their habitats are largely protected, while their scientific and economic relevance remains considerable. Advanced methods of in vitro cultivation provide the potential to work in a sustainable way with peat mosses and address aspects of basic research as well as biotechnological and economical topics like biomonitoring or the production of renewable substrates for horticulture (Sphagnum farming). Here, we describe the establishment of axenic in vitro cultures of the five peat moss species Sphagnum fimbriatum Wils. and Hook., Sphagnum magellanicum Brid., Sphagnum palustre L., Sphagnum rubellum Wils. and Sphagnum subnitens Russ. and Warnst. with specific focus on large-scale cultivation of S. palustre in bioreactors. Axenic, clonal cultures were established to produce high quantities of biomass under standardized laboratory conditions. For advanced production of S. palustre we tested different cultivation techniques, growth media and inocula, and analyzed the effects of tissue disruption. While cultivation on solid medium is suitable for long term storage, submerse cultivation in liquid medium yielded highest amounts of biomass. By addition of sucrose and ammonium nitrate we were able to increase the biomass by around 10- to 30-fold within 4 weeks. The morphology of in vitro-cultivated gametophores showed similar phenotypic characteristics compared to material from the field. Thus the tested culture techniques are suitable to produce S. palustre material for basic and applied research |
xmlui.metadata.dc.relation.publisherversion: | https://doi.org/10.1007/s11240-014-0658-2 |
xmlui.metadata.dc.identifier.uri: | http://hdl.handle.net/10347/24282 |
xmlui.metadata.dc.identifier.DOI: | 10.1007/s11240-014-0658-2 |
xmlui.metadata.dc.identifier.issn: | 0167-6857 |
xmlui.metadata.dc.identifier.e-issn: | 1573-5044 |
xmlui.metadata.dc.rights: | © The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited Atribución 4.0 Internacional |
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Except where otherwise noted, this item's license is described as © The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited