Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells
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Título: | Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells |
Autor/a: | Cabezas Sáinz, Pablo Coppel, Carlos Pensado López, Alba Fernández García, Pedro Muinelo Romay, Laura López López, Rafael Rubiolo Gaytán, Juan Andrés Sánchez Piñón, Laura Elena |
Centro/Departamento: | Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física |
Palabras chave: | Zebrafish | Development | Xenotransplantation | Incubation-temperature | Gene-expression | |
Data: | 2021 |
Editor: | MDPI |
Cita bibliográfica: | Genes 2021, 12(1), 113; https://doi.org/10.3390/genes12010113 |
Resumo: | Published studies show that most of the human cancer xenograft studies in zebrafish embryos have used incubation temperatures in the range of 32–34 °C for 3–6 days post-injection, trying to find a compromise temperature between the zebrafish embryos (28 °C) and the human injected cells (37 °C). While this experimental setup is widely used, a question remains: is possible to overcome the drawbacks caused by a suboptimal temperature for the injected cells? To clarify the effect of temperature and injected cells on the host, in this study, we analyzed the development and health of the last in response to different temperatures in the presence or absence of injected human cancer cells. Comparing different incubation temperatures (28, 34 and 36 °C), we determined morphological abnormalities and developmental effects in injected and non-injected embryos at different time points. Besides this, the expression of selected genes was determined by qPCR to determine temperature affected metabolic processes in the embryos. The results indicate that an incubation temperature of 36 °C during a period of 48 h is suitable for xenotransplantation without morphological or metabolic changes that could be affecting the host or the injected cells, allowing them to proliferate near their optimal temperature |
Versión do editor: | https://doi.org/10.3390/genes12010113 |
URI: | http://hdl.handle.net/10347/24464 |
DOI: | 10.3390/genes12010113 |
E-ISSN: | 2073-4425 |
Dereitos: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) Atribución 4.0 Internacional |
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