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dc.contributor.authorNeuparth, Teresa
dc.contributor.authorMachado, André M.
dc.contributor.authorMontes Goyanes, Rosa María
dc.contributor.authorRodil Rodríguez, María del Rosario
dc.contributor.authorBarros, Susana
dc.contributor.authorAlves, Nelson
dc.contributor.authorRuivo, Raquel
dc.contributor.authorCastro, Luis Filipe
dc.contributor.authorQuintana Álvarez, José Benito
dc.contributor.authorSantos, Miguel Machado
dc.date.accessioned2021-04-27T14:44:14Z
dc.date.available2021-04-27T14:44:14Z
dc.date.issued2020
dc.identifier.citationData in Brief Volume, 32 (2020), 106248
dc.identifier.issn2352-3409
dc.identifier.urihttp://hdl.handle.net/10347/26081
dc.description.abstractThe use of transcriptomics data brings new insights and works as a powerful tool to explore the molecular mode of action (MoA) of transgenerational inheritance effects of contaminants of emerging concern. Therefore, in this dataset, we present the transcriptomic data of the transgenerational effects of environmentally relevant simvastatin levels, one of the most prescribed human pharmaceuticals, in the keystone amphipod species Gammarus locusta. In summary, G. locusta juveniles were maintained under simvastatin exposure up to adulthood (exposed group - F0E) and the offspring of F0E were transferred to control water for the three subsequent generations (transgenerational group - F1T, F2T and F3T). To gain insights into the biological functions and canonical pathways transgenerationally disrupted by simvastatin, a G. locusta de novo transcriptome assembly was produced and the transcriptomic profiles of three individual G. locusta females, per group, over the four generations (F0 to F3) - solvent control groups (F0.C, F1.C, F2.C and F3.C), F0 320 ng/L simvastatin exposed group (F0.320E) and F1 to F3 320 transgenerational group (F1.320T; F2.320T and F3.320T) - were analyzed. Briefly, Illumina HiSeq™ 2500 platform was used to perform RNA sequencing, and due to the unavailability of G. locusta genome, the RNA-seq datasets were assembled de novo using Trinity and annotated with Trinotate software. After assembly and post-processing steps, 106093 transcripts with N50 of 2371 bp and mean sequence length of 1343.98 bp was produced. BUSCO analyses showed a transcriptome with gene completeness of 97.5 % Arthropoda library profile. The Bowtie2, RSEM and edgeR tools were used for the differential gene expression (DEGs) analyses that allowed the identification of a high quantity of genes differentially expressed in all generations. Finally, to identify the main metabolic pathways affected by the transgenerational effects of SIM across all generations, the DGEs genes were blasted onto KEGG pathways database using the KAAS webserver. The data furnished in this article allows a better molecular understanding of the transgenerational effects produced by simvastatin in the keystone amphipod G. locusta and has major implications for hazard and risk assessment of pharmaceuticals and other emerging contaminants. This article is related to the research article entitled “Transgenerational inheritance of chemical-induced signature: a case study with simvastatin
dc.description.sponsorshipThis article was developed under the Transobesogen project - Trans-phyletic obesogenic responses: from epigenetic modules to transgenerational environmental impacts (reference PTDC/CTA-AMB/31544/2017 - NORTE-01-0145-FEDER-031544), cofunded by Portugal 2020, the European Union through the ERDF and by the Portuguese Foundation for Science and Technology – FCT. This article was also supported by FCT through national funds (UIDB/04423/2020; UIDP/04423/2020), by the Spanish Agencia Estatal de Investigación (CTM2017-84763-C3-2-R) and by the Galician Council of Culture, Education and Universities (ED431C2017/36), cofounded by ERDF. A PhD grant awarded to Susana Barros acknowledges the doctoral grant attributed by FCT with reference PD/BD/143090/2018
dc.language.isoeng
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTM2017-84763-C3-2-R/ES/EVALUACION DEL EXPOSOMA DE CONTAMINANTES EMERGENTES EN AMBIENTES ACUATICOS
dc.rights© 2020 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectGammarus locusta
dc.subjectTransgenerational effects
dc.subjectRNA-Seq
dc.subjectTranscriptome analysis
dc.subjectDifferential gene expression
dc.subjectSimvastatin
dc.subjectMetabolic pathways
dc.titleTranscriptomic data on the transgenerational exposure of the keystone amphipod Gammarus locusta to simvastatin
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1016/j.dib.2020.106248
dc.relation.publisherversionhttps://doi.org/10.1016/j.dib.2020.106248
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Investigación e Análises Alimentarias
dc.description.peerreviewedSI


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© 2020 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Except where otherwise noted, this item's license is described as  © 2020 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)





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